| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,881,873 | A→T | 17.6% | Q163L (CAG→CTG) | oprN → | multidrug RND transporter outer membrane protein OprN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,881,873 | 0 | A | T | 17.6% | 63.7 / 5.8 | 28 | Q163L (CAG→CTG) | oprN | multidrug RND transporter outer membrane protein OprN |
| Reads supporting (aligned to +/- strand): ref base A (9/14); new base T (2/3); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCCCCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGTGG > NC_002947/3881724‑3882019 | gctCCCCAGCCAGACCACCGAGCGTGTCAATATCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACa < 1:563330/148‑1 (MQ=255) cccGGCCAGACCACCGAGCGGGGCAATAGCGCGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTg > 2:42781/1‑150 (MQ=255) ccGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGc < 1:35628/150‑1 (MQ=255) aGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCGGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCACGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGTcg < 1:763288/150‑2 (MQ=255) aGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGTc < 1:140713/140‑1 (MQ=255) aGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACTGTTGCAGGTc > 2:140713/1‑140 (MQ=255) ggTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGa < 2:562821/150‑1 (MQ=255) tCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAAc > 2:432290/1‑150 (MQ=255) cTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCaa > 1:421087/1‑150 (MQ=255) cTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACATGCAACAGTTGCAGGTCAGACTGATCGCCGAACTGGTCGATGCCTACGGGCAAATgcgc > 1:326852/1‑150 (MQ=255) tGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGcgcg < 2:421087/150‑1 (MQ=255) cATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGc < 1:432290/150‑1 (MQ=255) gAGCTTGACCTGTTCGGGCGCA‑CCAGTGACTAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTgcgc < 1:255/150‑1 (MQ=255) cTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATc > 2:448696/1‑150 (MQ=255) gggCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTg > 1:601006/1‑124 (MQ=255) gggCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTg < 2:601006/124‑1 (MQ=255) gggCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTg < 1:382648/150‑1 (MQ=255) ggCGCATCCAGCG‑CCAGATCGACGACCGAGATGTCCAGGAAGCGTTTGCCTCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTgg < 2:606282/150‑1 (MQ=255) tCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCcagc < 1:448696/150‑1 (MQ=255) cGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCcagca > 2:487760/1‑150 (MQ=255) gAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGa < 2:683309/80‑1 (MQ=255) gAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGa > 1:683309/1‑80 (MQ=255) gCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGGTGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGaa < 2:432534/150‑1 (MQ=255) gCCAGCGAAGCCCAAGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGTTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGaa < 1:37819/150‑1 (MQ=255) ggAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTAc < 1:52047/150‑1 (MQ=255) gAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTAcc < 1:487760/150‑1 (MQ=255) cAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTACGCGGTGCCCAGtt < 2:366524/68‑1 (MQ=255) cAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTACGCGGTGCCCAGtt > 1:366524/1‑68 (MQ=255) aaCAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGTgg > 1:723467/1‑150 (MQ=255) | GATCCCCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCG‑CCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGATGCGGGCGTGG > NC_002947/3881724‑3882019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |