Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,912,177 C→T 12.5% P160L (CCC→CTC)  PP_3452 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,912,1770CT12.5% 79.4 / 4.1 32P160L (CCC→CTC) PP_3452GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (14/14);  new base T (2/2);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGTCGAT  >  NC_002947/3912033‑3912319
                                                                                                                                                |                                                                                                                                              
cccTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGAt                                                                                                                                            <  1:381183/149‑1 (MQ=255)
cccTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGAt                                                                                                                                            >  2:381183/1‑149 (MQ=255)
cccTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATg                                                                                                                                           >  1:558583/1‑150 (MQ=255)
   tGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGGGCTCGATgcgg                                                                                                                                        <  1:660848/150‑2 (MQ=255)
               ggttggtGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCa                                                                                                                            <  1:268946/150‑1 (MQ=255)
                 ttggtGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATg                                                                                                                          <  1:141812/150‑1 (MQ=255)
                 ttggtGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATg                                                                                                                          >  1:629523/1‑150 (MQ=255)
                 gttgtGCTCGGGGTGTCCCCCGTGCTGTATGCCGTTTGGGTTCAGCCGGAGCTGCTCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATg                                                                                                                          <  2:606808/147‑1 (MQ=255)
                  tggtGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTCCCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGc                                                                                                                         <  1:118786/150‑1 (MQ=255)
                     tGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGAt                                                                                                                                            >  2:414546/1‑128 (MQ=255)
                     tGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGAt                                                                                                                                            <  1:414546/128‑1 (MQ=255)
                     tGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGa                                                                                                                      <  2:321271/150‑1 (MQ=255)
                     tGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGa                                                                                                                      <  2:321124/150‑1 (MQ=255)
                                  cACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCTGCATCGGGCTCGATgcgggtg                                                                                                                                     >  1:174434/1‑118 (MQ=255)
                                  cACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCTGCATCGGGCTCGATgcgggtg                                                                                                                                     <  2:174434/122‑5 (MQ=255)
                                  cACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCgt                                                                                                         >  1:734516/1‑150 (MQ=255)
                                            tATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGAGTTTCTAAGc                                                                                               >  1:83799/1‑150 (MQ=255)
                                               gCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGc                                                                                            <  2:734516/150‑1 (MQ=255)
                                                        gTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTcagc                                                                                   >  2:593835/1‑150 (MQ=255)
                                                                 gTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCagctacagctac                                                                          <  1:593835/150‑1 (MQ=255)
                                                                    cggcgCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCa                                                                                                                            <  2:263167/97‑1 (MQ=255)
                                                                    cggcgCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCa                                                                                                                            >  1:263167/1‑97 (MQ=255)
                                                                           ggTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATcc                                                                <  2:587817/150‑1 (MQ=255)
                                                                               aTTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCaa                                                            >  2:748937/1‑150 (MQ=255)
                                                                                                gcatcaCCCAGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCgtgt                                                                                                       <  2:266379/89‑1 (MQ=255)
                                                                                                gcatcaCCCAGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCgtgt                                                                                                       >  1:266379/2‑90 (MQ=255)
                                                                                                               tGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCACGGCCAAGCTGCACAATTTCTACCTGTCCAAgg                            >  1:685038/1‑150 (MQ=255)
                                                                                                                 cTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGAt                                                                                                                     <  1:775137/59‑1 (MQ=255)
                                                                                                                 cTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGAt                                                                                                                     >  2:775137/1‑59 (MQ=255)
                                                                                                                             tACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCaa              >  1:763659/1‑150 (MQ=255)
                                                                                                                                      cGCATCGGGCTCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGTc     >  2:9232/1‑150 (MQ=255)
                                                                                                                                         aTCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGTCGAt  >  1:716416/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGTCGAT  >  NC_002947/3912033‑3912319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑