| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,072,512 | G→C | 8.9% | A332G (GCG→GGG) | mdtA ← | multidrug transporter membrane fusion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,072,512 | 0 | G | C | 8.9% | 133.9 / 5.4 | 45 | A332G (GCG→GGG) | mdtA | multidrug transporter membrane fusion protein |
| Reads supporting (aligned to +/- strand): ref base G (16/25); new base C (2/2); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01 | |||||||||||
AGCACCAGACGGTCGCCGGCCTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGCGTGCCGGTGGTGGTGTCGATCTGG > NC_002947/4072365‑4072649 | aGCACCAGACGGTCGCCGGCCTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCcgcg > 2:513348/1‑150 (MQ=255) gACGGTCGCCGGCCTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGTCCGCGGCCggcg > 1:156120/1‑148 (MQ=255) ggTCGCCGGCCTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCTGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGccg < 2:548385/150‑2 (MQ=255) ccTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATCGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCt < 2:372415/150‑1 (MQ=255) cTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGa < 1:85551/143‑1 (MQ=255) cTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGa > 2:85551/1‑143 (MQ=255) cAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGttt < 2:674173/150‑1 (MQ=255) ccGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAgg < 2:306438/150‑1 (MQ=255) gagaATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGaccac < 1:101901/135‑1 (MQ=255) gagaATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGaccac > 2:101901/1‑135 (MQ=255) aaTGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGc < 2:162367/150‑1 (MQ=255) tGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCa > 1:55803/1‑150 (MQ=255) gACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGt > 2:152717/1‑133 (MQ=255) gACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGt < 1:152717/133‑1 (MQ=255) tcGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCCCGGCCGCGGCCAtcca < 1:198486/116‑4 (MQ=255) tcGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCCCGGCCGCGGCCAtcca > 2:198486/1‑113 (MQ=255) gCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGtt > 1:786008/1‑150 (MQ=255) cATCGCTGGCACGCACCTTCCGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGCCCATGACCACCTGTTTCAGGGTGTCGGCCAGCAAGCGCACCTTGa < 2:336172/150‑1 (MQ=255) cTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCCGCAAGCGCACGTTGAGGAAc > 2:95646/1‑150 (MQ=255) cTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAAc < 2:786008/150‑1 (MQ=255) tGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACt > 1:485725/1‑150 (MQ=255) cacccaccTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGt > 1:61649/1‑150 (MQ=255) cccaccTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTg > 2:604322/1‑150 (MQ=255) caccTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTggg < 2:485725/150‑1 (MQ=255) caccTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTggg < 2:549427/150‑1 (MQ=255) caccTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGNTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTggg < 1:604322/150‑1 (MQ=255) cTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGc < 2:55803/150‑1 (MQ=255) ttGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGAc < 1:329192/119‑1 (MQ=255) ttGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGAc > 2:329192/1‑119 (MQ=255) gACGGTGCTCTGCTCATTCACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGGGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGtt < 2:343830/150‑1 (MQ=255) aCGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTtc > 2:163972/1‑150 (MQ=255) ggTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTtctc < 1:785097/150‑1 (MQ=255) tctgctcATTGACAACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAAc > 2:631756/1‑150 (MQ=255) cATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCtt > 1:117792/1‑150 (MQ=255) tGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGa < 2:117792/150‑1 (MQ=255) tAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGc < 1:631756/150‑1 (MQ=255) gTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGCGTGCCggtg < 1:163972/150‑1 (MQ=255) ccATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGCGTGCCggtggtg < 2:61649/150‑1 (MQ=255) cATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTggg < 1:334154/91‑1 (MQ=255) cATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTggg > 2:334154/1‑91 (MQ=255) cATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGaaa > 1:21729/1‑119 (MQ=255) cATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGaaa < 2:21729/119‑1 (MQ=255) gCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGCGTGCCGGTGGTGGTGTCGAt < 1:396303/150‑1 (MQ=255) aaCTGGATGGCCGCGGCCGGGGCCATGACCGCCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGCGTGCCGGTGGTGGTGTCGATCTgg < 1:592672/150‑1 (MQ=255) aTGGCCCCGGCCGCGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCttt > 1:347256/1‑110 (MQ=255) aTGGCCCCGGCCGCGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCttt < 2:347256/110‑1 (MQ=255) | AGCACCAGACGGTCGCCGGCCTTCAAGCCGTCGAGAATGACGCTGTTCTCGCCATCGCTGGCACCCACCTTCAGCTTGCGGACATTGACGGTGCTCTGCTCATTGACCACATAGGCGAAGGTGCCATCGTTGCCAAACTGGATGGCCGCGGCCGGGGCCATGACCACCTGTTTGAGGGTGTCGGCCAGCAAGCGCACGTTGACGAACTGGTTGGGGAACAGGGCCAGGTCCTTGTTCTCGAAACGGCCTTTGAACTTCAGCGTGCCGGTGGTGGTGTCGATCTGG > NC_002947/4072365‑4072649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |