| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,120,891 | G→A | 10.3% | A421A (GCG→GCA) | adhB → | alcohol dehydrogenase cytochrome c subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,120,891 | 0 | G | A | 10.3% | 103.0 / 3.9 | 39 | A421A (GCG→GCA) | adhB | alcohol dehydrogenase cytochrome c subunit |
| Reads supporting (aligned to +/- strand): ref base G (18/17); new base A (2/2); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01 | |||||||||||
TATCACGCTCAATGGTTCGCAGCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCCAACAAAGACCGCGCAGCACATGAGCCAC > NC_002947/4120744‑4121037 | tatCACGCTCAATGGTTCGCAGCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGt < 2:238211/150‑1 (MQ=255) aCGCTCAATGGTTCGCAGCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGAt > 2:107107/1‑150 (MQ=255) cAATGGTTCGCAGCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCa > 2:324953/1‑150 (MQ=255) cAGCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGc > 2:695796/1‑150 (MQ=255) gCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCaa < 1:273391/150‑1 (MQ=255) gCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATAGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCaa < 1:695796/150‑1 (MQ=255) ggtggtGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCt < 1:324953/150‑1 (MQ=255) tggCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTg > 2:761497/1‑150 (MQ=255) tggCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTg < 1:540127/150‑1 (MQ=255) ggCTGGAGGGGTTCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTgg < 2:643269/150‑1 (MQ=255) cGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGGCGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCcag < 1:761497/150‑1 (MQ=255) aTCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCcagcagca > 1:622491/1‑150 (MQ=255) cGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCagcagcagc < 2:196132/150‑1 (MQ=255) cGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTatca > 2:254319/1‑150 (MQ=255) ggCTTTGTGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTatcat > 1:468812/1‑150 (MQ=255) gcgcGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCa > 2:586257/1‑150 (MQ=255) gTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAAttc < 2:546776/150‑2 (MQ=255) tCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGAAGACCTAGCTTGGTGCATATGCGTTGAATTGGAGt > 2:498388/1‑150 (MQ=255) tCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTACGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGt > 2:263425/1‑150 (MQ=255) aGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCAGTGGATGCGCAGGCGGTAGGCAAGCTGCGTgg < 1:695028/91‑1 (MQ=255) aGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCAGTGGATGCGCAGGCGGTAGGCAAGCTGCGTgg > 2:695028/1‑91 (MQ=255) gagaTTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGca > 2:4242/1‑150 (MQ=255) gagaTTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGca > 2:55307/1‑150 (MQ=255) gagaTTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTACATATGCGTTGAATTGGAGTGGca < 1:254319/150‑1 (MQ=255) cGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGc > 2:711936/1‑125 (MQ=255) cGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGc < 1:711936/125‑1 (MQ=255) tGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCAGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGa < 1:677444/131‑1 (MQ=255) tGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCAGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGa > 2:677444/1‑131 (MQ=255) cTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGa > 2:595668/1‑150 (MQ=255) tttGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCcagcagca > 1:67678/1‑91 (MQ=255) tttGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCcagcagca < 2:67678/91‑1 (MQ=255) cGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCcaa < 1:464469/150‑1 (MQ=255) gTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCcaac > 1:354524/1‑150 (MQ=255) gTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATAATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGa > 1:378181/1‑137 (MQ=255) gTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATAATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGa < 2:378181/137‑1 (MQ=255) gCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCCAACAAAGa < 1:595668/150‑1 (MQ=255) gggCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCCAACAAAGACcgcg > 1:236784/1‑150 (MQ=255) gTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCCAACAAAGACCGCGCAGCACATGAGCCa < 1:586257/150‑1 (MQ=255) tGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCGTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCCAACAAAGACCGCGCAGCACATGAGCCAc < 2:622491/150‑1 (MQ=255) | TATCACGCTCAATGGTTCGCAGCGGGTGGTGGCTGCAGGGGTGCCGGACGCCTATCGCATGCCGGCTTTGCGCGAGCAGTTGAGTGATCAGGAGATTGCCGACGTGCTCAGCTTTGTGCGTACGGCCTGGGGCAACCAGGGGGGTGCGGTGGATGCGCAGGCGGTAGGCAAGCTGCGTGGGCATACCGACCCGGCCAGCAGCAGCCCTATCATCTTGCATATGCGTTGAATTGGAGTGGCACATGTACCGACGCGAGGCTGCCCCCAACAAAGACCGCGCAGCACATGAGCCAC > NC_002947/4120744‑4121037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |