| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,163,553 | G→A | 18.5% | intergenic (‑48/‑134) | pssA ← / → PP_3665 | CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,163,553 | 0 | G | A | 18.5% | 53.3 / 7.5 | 27 | intergenic (‑48/‑134) | pssA/PP_3665 | CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (8/14); new base A (3/2); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01 | |||||||||||
GAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCG > NC_002947/4163404‑4163684 | gAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCcggcg > 2:564977/1‑150 (MQ=255) aaaaggcacgcGCGGTAAGCCCTGCCATTGGGCAGCACCGGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCcggcgg < 2:644228/147‑1 (MQ=255) aCAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg < 1:308760/150‑1 (MQ=255) gccggcGCGGTAAGCCCTGGCATCGGGCAGCCCCTGGATCGCTGCGGGGGCCATGGCGTAGCCTGGCACACCCACCCGCATGGTTTCGGCATTCGCTGACAGCCACCTTGGGCGTGCAACCACGGTCGTCGCGGCCGGCCGTCGGGGCCa < 2:8211/147‑1 (MQ=255) cgcgGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCt < 2:699679/150‑1 (MQ=255) gcgGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACACTCCGCCCTGCCTGCCGGCGGGGCCATTCCTg < 1:619677/150‑1 (MQ=255) aaGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGc < 1:564977/150‑1 (MQ=255) aGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCt < 2:235548/150‑1 (MQ=255) gCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGc > 1:189619/1‑135 (MQ=255) gCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGc < 2:189619/135‑1 (MQ=255) tGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATc > 1:797554/1‑150 (MQ=255) cGGGCAGCACCGGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGCGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGgt < 1:432272/150‑1 (MQ=255) gggCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGccga < 1:334979/127‑3 (MQ=255) gggCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGccga > 2:334979/1‑125 (MQ=255) gcagcaCCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTa < 1:156018/150‑1 (MQ=255) aCCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGgtgcttgagc < 2:74227/150‑8 (MQ=255) gCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCAt > 1:131275/1‑150 (MQ=255) aTGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCgggg < 1:591971/120‑1 (MQ=255) aTGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCgggg > 2:591971/1‑120 (MQ=255) gCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGaa < 2:566107/150‑1 (MQ=255) tGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATc > 2:589261/1‑118 (MQ=255) tGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATc < 1:589261/118‑1 (MQ=255) cAGCATGGTTTCGGGGTCCACGGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTg < 1:438803/150‑1 (MQ=255) tttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGaa < 2:209492/127‑1 (MQ=255) tttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGaa > 1:209492/1‑127 (MQ=255) ggggTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCAATgg > 1:476427/1‑149 (MQ=255) tGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGgtgt < 2:212713/79‑1 (MQ=255) tGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGgtgt > 1:212713/1‑79 (MQ=255) aCTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAAc > 2:603907/1‑150 (MQ=255) atggCCCCGCCGGCAGGCAGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAgcg > 1:653097/3‑150 (MQ=255) | GAACAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCG > NC_002947/4163404‑4163684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |