Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	26,844	A→T	9.8%	intergenic (+40/+145)	PP_0021 → / ← PP_0022	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	26,844	0	A	T	9.8%	104.6 / 5.1	41	intergenic (+40/+145)	PP_0021/PP_0022	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/19);  new base T (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-01

TCGAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCTATA  >  NC_002947/26698‑26993                                                                                                                                                    |                                                                                                                                                      tCGAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTg                                                                                                                                                    <  2:360472/150‑1 (MQ=255)     aTCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTGTCACTGT‑AACCACAGTGGttt                                                                                                                                                >  2:409966/1‑150 (MQ=255)            aTCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGGttaca                                                                                                                                              >  2:125555/1‑143 (MQ=255)            aTCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGGttaca                                                                                                                                              <  1:125555/146‑4 (MQ=255)                  aTGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaaa                                                                                                                                   <  2:224358/150‑1 (MQ=255)                      cGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTgg                                                                                                                               <  1:409966/150‑1 (MQ=255)                                tcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGt                                                                                                                     >  1:103483/1‑150 (MQ=255)                                   ttATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGc                                                                                                                  >  2:199627/1‑150 (MQ=255)                                         cAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGac                                                                                                            >  1:544552/1‑150 (MQ=255)                                             aTGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACAcgc                                                                                                        <  1:274750/150‑1 (MQ=255)                                                  tAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCt                                                                                                   >  2:148758/1‑150 (MQ=255)                                                     agTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc                                                                                                <  1:304005/150‑1 (MQ=255)                                                       tGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCa                                                                                              <  1:148758/150‑1 (MQ=255)                                                                 ccGGGAGGAACTGGTCGTTCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc                                                                                    <  1:610974/150‑1 (MQ=255)                                                                        gaACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAgcgcg                                                                             <  1:199627/150‑1 (MQ=255)                                                                           cTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaa                                                                                                                                    <  2:48370/92‑1 (MQ=255)                                                                           cTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaa                                                                                                                                    >  1:48370/1‑92 (MQ=255)                                                                                         ggAGCTGCATCCGGTCTGGAGATGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt                                                            <  1:124625/150‑1 (MQ=37)                                                                                             cTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGt                                                        >  1:44636/1‑150 (MQ=255)                                                                                                  tCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGc                                                   >  1:787134/1‑150 (MQ=255)                                                                                                  tCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGc                                                   >  1:787127/1‑150 (MQ=255)                                                                                                      gCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTc                                                <  2:794082/149‑1 (MQ=255)                                                                                                      gCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTc                                                >  1:794082/1‑149 (MQ=255)                                                                                                        cTGAGTAGTCCGGTTTTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGaa                                             <  2:44636/150‑1 (MQ=255)                                                                                                        cTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGaa                                             <  2:103483/150‑1 (MQ=255)                                                                                                                gCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTAtg                                     >  1:327269/1‑150 (MQ=255)                                                                                                                   tgttgtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCGCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTAtgctg                                  >  1:314305/1‑150 (MQ=255)                                                                                                                    gttgtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTAtgctgc                                 >  1:18512/1‑150 (MQ=255)                                                                                                                       gtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaaa                                                                                                                                   >  1:748460/1‑49 (MQ=255)                                                                                                                       gtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaaa                                                                                                                                   <  2:748460/49‑1 (MQ=255)                                                                                                                                tttttttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGTTCGGGGCCTTga                     >  1:50375/1‑149 (MQ=255)                                                                                                                                   ttttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCAc                  >  2:38730/1‑150 (MQ=255)                                                                                                                                     ttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAacac                                          >  1:586187/1‑124 (MQ=255)                                                                                                                                     ttCACTGT‑AACCACAGTGGTTTAAAGTGAATAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAacac                                          <  2:586187/124‑1 (MQ=255)                                                                                                                                      tCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc                                                                                                <  1:726563/69‑1 (MQ=255)                                                                                                                                      tCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc                                                                                                >  2:726563/1‑69 (MQ=255)                                                                                                                                        aCTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCAtt                                                                    <  2:335522/95‑1 (MQ=25)                                                                                                                                        aCTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCAtt                                                                    >  1:335522/1‑95 (MQ=25)                                                                                                                                           gT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgt          <  2:787127/150‑1 (MQ=255)                                                                                                                                           gT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgt          <  2:787134/150‑1 (MQ=255)                                                                                                                                           gT‑AACCACAGTGGGTTAAAGTAAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgt          <  2:272959/150‑1 (MQ=255)                                                                                                                                                 cacaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCt     >  2:367208/1‑150 (MQ=255)                                                                                                                                                    aGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCtata  >  2:277049/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TCGAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCTATA  >  NC_002947/26698‑26993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑