| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,221,235 | A→C | 21.8% | intergenic (+50/+212) | PP_3699 → / ← PP_3700 | hypothetical protein/chromosome partitioning ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,221,235 | 0 | A | C | 21.8% | 31.7 / 9.9 | 23 | intergenic (+50/+212) | PP_3699/PP_3700 | hypothetical protein/chromosome partitioning ATPase |
| Reads supporting (aligned to +/- strand): ref base A (10/8); new base C (2/3); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01 | |||||||||||
TTGCGTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCATCTCTAGCTCCGAGCA > NC_002947/4221089‑4221360 | ttGCGTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTTAAGNTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAATTGAAGTGGCAGATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGAt > 1:131213/1‑150 (MQ=255) aaCGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGccccc > 1:103243/1‑147 (MQ=255) aaCGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGccccc < 2:103243/148‑2 (MQ=255) aCGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGccccctga < 1:318125/150‑5 (MQ=255) gTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctc > 2:140882/1‑150 (MQ=255) aCGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctcagcctcagc < 1:214535/150‑1 (MQ=255) tGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCaaaa < 1:394255/150‑1 (MQ=255) gATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTa < 1:140882/150‑1 (MQ=255) tCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctca < 2:701615/109‑1 (MQ=255) tCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctca > 1:701615/1‑109 (MQ=255) aGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGcc > 1:343497/1‑150 (MQ=255) aGGGCTGATATGCCTGAGGAGTTTGACCAAGCCTGCAAGTGAAGTGGCAAATGCTGACCGTAGCGCCTAGCGCGGTCAGGAGGCATCGGACAGACGCCCCACCCTCAGCCTCCGCCCCCGCCTCAAACCCGATCTACGGCCGAGCGAGcc > 2:59436/1‑150 (MQ=255) atatGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCca > 2:398459/1‑150 (MQ=255) gTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGccccctga < 2:786203/84‑5 (MQ=37) gTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGccccctga > 1:786203/1‑80 (MQ=37) aCAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTg > 1:66820/1‑150 (MQ=255) gCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGa < 1:398459/150‑1 (MQ=255) agtgaagtgGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGccc < 2:451996/62‑1 (MQ=255) agtgaagtgGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGccc > 1:451996/1‑62 (MQ=255) tGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCAtct > 1:110244/1‑150 (MQ=255) tAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCATCTCTAGCTCCGAGCa < 2:678540/150‑1 (MQ=255) tCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGa < 1:494318/109‑1 (MQ=255) tCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGa > 2:494318/1‑109 (MQ=255) | TTGCGTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCATCTCTAGCTCCGAGCA > NC_002947/4221089‑4221360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |