| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,224,546 | T→G | 16.1% | intergenic (‑614/+207) | PP_3703 ← / ← PP_3704 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,224,546 | 0 | T | G | 16.1% | 63.6 / 9.3 | 31 | intergenic (‑614/+207) | PP_3703/PP_3704 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (11/15); new base G (2/3); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01 | |||||||||||
GTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCAAGGAAGGTTCTGTGGAGAAGGGA > NC_002947/4224397‑4224692 | gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACGACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGTAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTCAGTGGC‑‑CCAGCAGTGCCCCt > 2:578264/1‑150 (MQ=255)gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCt < 2:719692/150‑1 (MQ=255) ctcccTATCAATCCAAACGTAAATGTCCCTAAGCCTGCTTGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCtgt < 1:209413/148‑1 (MQ=255) ccTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGACAACCCAGCGCCAGAGACACCTAGGAGGC‑‑CCAGCAGTGCACCtgtgag > 2:209413/1‑148 (MQ=255) ccTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCa < 1:110271/150‑1 (MQ=255) aaTCTAAACGTAAATTTCCTTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATATGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAATGCACAGGTGCActgctg < 1:371348/150‑1 (MQ=255) tCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTg < 2:581353/145‑1 (MQ=255) tCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTg > 1:581353/1‑145 (MQ=255) aaaCGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAATGCACAGGTGCACTGCTgg < 2:364068/146‑1 (MQ=255) aaaCGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAATGCACAGGTGCACTGATgg > 1:364068/1‑146 (MQ=255) gactaaTGGCTCTAGGCCTGACCCCGGGGAACAACCTCCGGCCTGCAACAAACAGGGCGAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCAc < 2:351301/146‑1 (MQ=255) cTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCt > 1:357274/1‑150 (MQ=255) aaGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTa < 2:301045/150‑1 (MQ=255) aGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTAt < 1:95762/150‑1 (MQ=255) gCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCAACCAGCAATGCACAGGTGCACTGCt > 1:162537/1‑128 (MQ=38) gCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCAACCAGCAATGCACAGGTGCACTGCt < 2:162537/128‑1 (MQ=38) ccTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGTCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa < 1:652106/150‑1 (MQ=255) ggggAACAACCTCGCTCCCGTAACAAAGACGGCCAGATCCGTACTACCAGGAGGTATGCGGGAAAACCCCGCGAAAAAGTAACCTAGGTGGG‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGAc < 1:776391/150‑1 (MQ=255) ggggAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGAc < 1:106211/150‑1 (MQ=255) tCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTa > 1:442606/1‑150 (MQ=255) tcgcGTAACAAAGACGGCCAGATAAGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATcgc < 2:395528/147‑1 (MQ=255) gCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGTCTTTCGCGCCCTTCTGTAGCGga < 2:272283/150‑1 (MQ=255) gCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGga < 2:357274/150‑1 (MQ=255) ccAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGtt < 2:144924/88‑1 (MQ=255) ccAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGtt > 1:144924/1‑88 (MQ=255) gTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACa > 1:445950/1‑150 (MQ=255) gATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGa > 2:554421/1‑150 (MQ=255) aTAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGaa < 2:445950/150‑1 (MQ=255) cTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCaagga > 2:53266/1‑150 (MQ=255) gC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCAAGGAAGGTTCt > 1:183853/1‑150 (MQ=255) ccAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCt > 2:37243/1‑52 (MQ=255) ccAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCt < 1:37243/52‑1 (MQ=255) gCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGga < 1:37903/81‑1 (MQ=255) gCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGga > 2:37903/1‑81 (MQ=255) aCCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCAAGGAAGGTTCTGTGGAGAAGGGa > 2:42983/1‑150 (MQ=255) | GTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGC‑‑CCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCAAGGAAGGTTCTGTGGAGAAGGGA > NC_002947/4224397‑4224692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |