Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,281,403 G→A 14.9% T22I (ACC→ATC)  PP_3751 ← cyanate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,281,4030GA14.9% 56.5 / 2.4 26T22I (ACC→ATC) PP_3751cyanate transporter
Reads supporting (aligned to +/- strand):  ref base G (9/13);  new base A (2/2);  total (11/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

CGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAGAGATTGGAT  >  NC_002947/4281254‑4281544
                                                                                                                                                     |                                                                                                                                             
cgccATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGGTCGAGTAACTGGCCGATACTgg                                                                                                                                               >  2:178228/1‑150 (MQ=255)
   cATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCa                                                                                                                                            <  2:30765/150‑1 (MQ=255)
     tGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAgg                                                                                                                                          <  2:702301/150‑1 (MQ=255)
               cccAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCa                                                                                                                                <  1:176163/150‑1 (MQ=255)
                       cGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGCCACTGGTCAGGATCGGCCGCAGGTTCAAc                                                                                                                        <  1:146043/150‑1 (MQ=255)
                       cGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAAc                                                                                                                        <  2:324692/150‑1 (MQ=255)
                        gCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACg                                                                                                                       >  1:713270/1‑150 (MQ=255)
                                      aaCAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCAcc                                                                                                         >  1:73560/1‑150 (MQ=255)
                                             gccccAGGCCCATCCACAGCACCGGTAGCGCCGTTAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCAGGCCTTTATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACa                                                                                                  <  1:178228/147‑1 (MQ=255)
                                               aCCAGGCCTATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAgg                                                                                                <  2:301375/150‑1 (MQ=255)
                                                   ggCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCa                                                                                            <  2:713270/150‑1 (MQ=255)
                                                   ggCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATACCCAGCAAGAGGGTCa                                                                                            >  2:170430/1‑150 (MQ=255)
                                                      ccATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTa                                                                                         >  2:433277/1‑150 (MQ=255)
                                                                 aCCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCAtt                                                                              <  1:274900/150‑1 (MQ=255)
                                                                    ggTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATtgtg                                                                           >  2:705347/1‑150 (MQ=255)
                                                                        gcgcCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTc                                                                       >  1:465067/1‑150 (MQ=255)
                                                                         cgcCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCg                                                                      <  1:705347/150‑1 (MQ=255)
                                                                           ccGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGt                                                                    <  1:433277/150‑1 (MQ=255)
                                                                                  cAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTg                                                             <  2:109964/150‑1 (MQ=255)
                                                                                                      ccAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGGTCAGTAGAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTAt                                         >  1:445742/1‑150 (MQ=255)
                                                                                                                              gTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCa                    >  1:758817/1‑147 (MQ=255)
                                                                                                                              gTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCa                    <  2:758817/147‑1 (MQ=255)
                                                                                                                                      ctgcggCCGATCCTGACCAGTATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTaa                                                          >  2:201120/5‑101 (MQ=38)
                                                                                                                                      ctgcggCCGATCCTGACCAGTATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTaa                                                          <  1:201120/97‑1 (MQ=38)
                                                                                                                                       tgcggCCGATCCTGACCAGTATCGGCCACAGGTTCAACGCCAGCGGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAgaga        >  1:463707/4‑150 (MQ=255)
                                                                                                                                            ccGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAGAGATTGGa   >  2:336500/1‑150 (MQ=255)
                                                                                                                                             cGATACTGATCAGCATCGGCCGCAGTTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAGAGATTGGAt  <  2:55074/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
CGCCATGCTCGCTGACCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAGAGATTGGAT  >  NC_002947/4281254‑4281544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑