| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,297,638 | G→A | 10.2% | R154C (CGC→TGC) | PP_3768 ← | shikimate 5‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,297,638 | 0 | G | A | 10.2% | 129.5 / 6.2 | 49 | R154C (CGC→TGC) | PP_3768 | shikimate 5‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (20/24); new base A (2/3); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
TCGGGAAACGCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGGTG > NC_002947/4297490‑4297786 | tCGGGAAACGCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCACATGCCGCGCGTCTCAAGCACCCAGCGATAACCACACACATCCGCCCCCTGACGCCCGGCCTGCTCGTTGCGt > 1:216026/1‑150 (MQ=255)tCGGGAAACGCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGt < 2:390458/150‑1 (MQ=255) cAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTAACGCCCGGCCTGCTCGTTGCGTGCAACgatgat < 2:541011/150‑1 (MQ=255) ctctTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgagcaggcc < 2:62736/150‑8 (MQ=255) tcttctGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCCCCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgat < 1:496072/143‑1 (MQ=255) tcttctGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgat > 2:496072/1‑143 (MQ=255) cTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgatgat > 1:710187/1‑138 (MQ=255) cTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgatgat < 2:710187/138‑1 (MQ=255) cAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAAccc > 1:492876/1‑150 (MQ=255) ccGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgat > 1:38076/1‑128 (MQ=255) ccGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgat < 2:38076/128‑1 (MQ=255) gCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCgcgc < 2:210932/150‑1 (MQ=255) ccATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCa > 2:289560/1‑150 (MQ=255) cATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCaa > 2:76470/1‑150 (MQ=255) cATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCaa < 2:415850/150‑1 (MQ=255) aTACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAg > 2:70928/1‑150 (MQ=255) gATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCg < 1:660195/124‑1 (MQ=255) gATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCg > 2:660195/1‑124 (MQ=255) gCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgagca > 2:111338/1‑108 (MQ=255) gCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgagca < 1:111338/111‑4 (MQ=255) gTTCCCCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGCCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCtt < 1:564249/150‑1 (MQ=255) gTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCtt > 1:364575/1‑150 (MQ=255) gCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGc > 1:674473/1‑150 (MQ=255) aTGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAGCTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCa > 1:635267/1‑150 (MQ=255) gggcgggcAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCAcc > 1:148349/1‑150 (MQ=255) gcgggcAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGc > 1:517561/1‑150 (MQ=255) gTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAg < 2:110344/150‑1 (MQ=255) gTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAg < 2:148349/150‑1 (MQ=255) ccTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgag < 1:631684/74‑2 (MQ=255) ccTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgag > 2:631684/1‑73 (MQ=255) cTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGc < 1:289560/150‑1 (MQ=255) ggCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGTAAAGGCCGTg < 2:517561/150‑1 (MQ=255) cAGCGATAACCACAGACATCCGCCAACTGGCGCCCGGCCTGCTCGTTGCGGGCAACGATGATGGCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGGCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGa < 1:76196/150‑1 (MQ=255) cAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCgg < 2:296043/79‑1 (MQ=255) cAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCgg > 1:296043/1‑79 (MQ=255) aTAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGAc < 2:364575/150‑1 (MQ=255) aTAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGAc < 1:748549/150‑1 (MQ=255) ccACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGAt > 2:333677/1‑142 (MQ=255) ccACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGAt < 1:333677/142‑1 (MQ=255) ccACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGa > 2:315795/1‑150 (MQ=255) aCATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGa < 2:305564/150‑1 (MQ=255) gCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGAc > 1:618327/1‑134 (MQ=255) gCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGAc < 2:618327/134‑1 (MQ=255) aCTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAAtt > 1:442715/1‑150 (MQ=255) tGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAACGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTgg < 1:499093/150‑1 (MQ=255) ccTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCa < 1:244926/150‑1 (MQ=255) atcatCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGa > 1:380799/5‑112 (MQ=255) atcatCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGa < 2:380799/108‑1 (MQ=255) cGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGgtg < 1:569761/150‑1 (MQ=255) gTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGAt > 1:46832/1‑111 (MQ=255) gTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGAt < 2:46832/111‑1 (MQ=255) | TCGGGAAACGCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGGTG > NC_002947/4297490‑4297786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |