| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 205,616 | G→T | 8.9% | E3708* (GAA→TAA) ‡ | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 205,616 | 0 | G | T | 8.9% | 132.0 / 3.6 | 45 | E3708* (GAA→TAA) ‡ | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (21/20); new base T (2/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
CGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGA > NC_002947/205474‑205746 | cGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTggt > 2:624564‑M2/1‑147 (MQ=255) gCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTggtg > 2:703198‑M2/1‑146 (MQ=255) gCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTggtg < 1:41817‑M2/150‑5 (MQ=255) cTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTggtgg > 2:41817‑M2/1‑145 (MQ=255) cTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTggtgg > 2:507438‑M2/1‑145 (MQ=255) gATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGaa > 2:242943/1‑132 (MQ=40) gATCACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGaa < 1:242943/132‑1 (MQ=255) gTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGAcc > 1:713552/1‑150 (MQ=255) tGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGAGGTGGCCGTGCTGACCGCGACCCCCTCGGTCAc > 2:498704/1‑150 (MQ=255) gCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGt < 2:732734/138‑1 (MQ=255) gCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGt > 1:732734/1‑138 (MQ=255) cAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggc > 1:599861/1‑150 (MQ=255) aGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcg < 2:676376/150‑1 (MQ=40) cAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCga < 1:498704/150‑1 (MQ=40) gggTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc < 2:599861/150‑1 (MQ=255) cAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTacac < 2:713552/150‑1 (MQ=255) gTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTg > 1:684598/1‑150 (MQ=40) gATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTc > 2:527437/1‑107 (MQ=38) gATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTc < 1:527437/107‑1 (MQ=38) gATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGaa < 2:223482/117‑1 (MQ=37) gATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGaa > 1:223482/1‑117 (MQ=37) ggctaCTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACcaa < 1:649022/146‑1 (MQ=25) ccTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGc > 1:231084/1‑150 (MQ=34) cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACC‑ACTTCGTcggtggtgtcggtcact < 2:190020‑M2/79‑18 (MQ=255) cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACC‑ACTTCGTcggtggtgtcggtcact < 2:587127‑M2/79‑18 (MQ=255) cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACC‑ACTTCGTcggtggtgtcggtcact > 1:587127‑M2/1‑62 (MQ=255) cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACC‑ACTTCGTcggtggtgtcggtcact > 1:190020‑M2/1‑62 (MQ=255) ttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAg > 1:731378/1‑100 (MQ=34) ttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAg < 2:731378/100‑1 (MQ=34) ccTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGcc < 1:653642/141‑1 (MQ=34) ccTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGcc > 2:653642/1‑141 (MQ=35) aCGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCt < 2:684598/150‑1 (MQ=39) cTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCa < 2:231084/150‑1 (MQ=37) aaGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAg > 1:409274/1‑102 (MQ=38) aaGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAg < 2:409274/102‑1 (MQ=38) cGACACCACCGACGAAGTggtggtgtcggtcacttgaaccgaagcgttgccgcccagttgcaggttctcgaaggtcgcaccagtggcatc < 2:497538‑M2/90‑73 (MQ=255) cGACACCACCGACGAAGTggtggtgtcggtcacttgaaccgaagcgttgccgcccagttgcaggttctcgaaggtcgcaccagtggcatc > 1:497538‑M2/1‑18 (MQ=255) gACACCACCGACGAAGTggtggtgtcggtcacttgaaccgaagcgttgccgcccagttgcaggttctcgaaggtcgcaccagtggcatccaccgccgagttgatacccaggctgatctggccagcatcgttgtagacgtcatc > 1:522687‑M2/1‑17 (MQ=255) gACACCACCGACGAAGTggtggtgtcggtcacttgaaccgaagcgttgccgcccagttgcaggttctcgaaggtcgcaccagtggcatccaccgccgagttgatacccaggctgatctggccagcatcgttgtagacgtcatc < 2:522687‑M2/143‑127 (MQ=255) acacCACCGACGCAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGc > 2:314515/1‑140 (MQ=21) acacCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGc < 1:314515/140‑1 (MQ=35) accaccGACGAAGTggtggtgtcggtgacttgaaccgaagcgttg < 2:635432‑M2/45‑32 (MQ=255) accaccGACGAAGTggtggtgtcggtgacttgaaccgaagcgttg > 1:635432‑M2/1‑14 (MQ=255) caccGACGAAGTggtggtgtcggtcacttgaaccgaagcgttgccgcccagttgcaggttctcgaaggtcgcaccagtg < 2:54947‑M2/79‑68 (MQ=255) caccGACGAAGTggtggtgtcggtcacttgaaccgaagcgttgccgcccagttgcaggttctcgaaggtcgcaccagtg > 1:54947‑M2/1‑12 (MQ=255) | CGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGA > NC_002947/205474‑205746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |