| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,339,035 | T→A | 10.3% | L33Q (CTG→CAG) | ribAB‑II → | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,339,035 | 0 | T | A | 10.3% | 112.6 / 5.2 | 39 | L33Q (CTG→CAG) | ribAB‑II | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (20/15); new base A (2/2); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
TATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGCCAACGGTAGCGTGT > NC_002947/4338891‑4339178 | tATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGACCGATctgctca > 2:442364/1‑148 (MQ=255) ttCACCCCACCACAGTGAGCGTGCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGAc > 1:472074/1‑150 (MQ=255) cccACCACAGTGAGCGTGCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCa > 2:455618/1‑150 (MQ=255) gTGCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCAt < 1:269682/150‑1 (MQ=255) gCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGCGGACCGGGAAAACGAGGGCGATCTGCTGATCgccgcc < 1:645095/116‑1 (MQ=255) gCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCgccgcc > 2:645095/1‑116 (MQ=255) tttCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTGCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGgc > 1:618394/1‑150 (MQ=255) tttCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAGCGAGGGCGATCAGCAGATcgccct > 2:717895/1‑103 (MQ=255) tttCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCAGCAGATcgccct < 1:717895/105‑3 (MQ=255) aaCACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCAGCAGATcgccct < 2:24426/101‑3 (MQ=255) aaCACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCAGCAGATcgccct > 1:24426/1‑99 (MQ=255) acacCATCGAAGAATTTCTCGAGGACCACCGGCAAGGCAAGATGGAACTACTGGTGCATGACGAGGACCGGGGAAACGAGGGCGATCTGCGGATCGCCGCCGAGCGCTGCGACGCCCAGGCGATATACTTGATGGCGAGCGAGTcgcgcg > 2:311516/1‑150 (MQ=255) aTCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCAt < 1:11435/119‑1 (MQ=255) aTCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCAt > 2:11435/1‑119 (MQ=255) tCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGAc < 1:408053/107‑1 (MQ=255) tCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGAc > 2:408053/1‑107 (MQ=255) gAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATc > 1:754716/1‑150 (MQ=255) gAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCCCTCCCACCAACAAGCAATCATCTTCATCGGACGTGAGGTCCGTGGTCTGATCTGc < 2:446677/150‑1 (MQ=255) ctcGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGTGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGAcc < 2:225809/150‑1 (MQ=255) tcGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCt > 1:12389/1‑150 (MQ=255) tACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACcgac < 1:442364/150‑1 (MQ=255) tACCGGCAAGGCAAGATGGTCCTGCTGGTGGAGGACTAGGACCGGGAAAACGAGGGCGACCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCCCCCGAGGCGCGCGGGCTGATCTGCCTCACCTTGACctac > 2:76126/1‑150 (MQ=255) ggcaaggcaaGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATc < 2:286924/65‑1 (MQ=255) ggcaaggcaaGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATc > 1:286924/1‑65 (MQ=255) aggcaagATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTg > 2:536194/1‑150 (MQ=255) ggcaagATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGc > 1:426146/1‑150 (MQ=255) aTGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAgcgc > 1:591520/1‑150 (MQ=255) tCCTGCTGATAGACGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCgcctgg < 2:618394/150‑1 (MQ=255) ctgGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCgcctgggcctg < 2:754716/150‑1 (MQ=255) aCGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCgcct < 2:117279/133‑1 (MQ=255) aCGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCgcct > 1:117279/1‑133 (MQ=255) aCGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATgg < 2:472074/150‑1 (MQ=255) gACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCg < 2:603982/150‑1 (MQ=255) cGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGcc > 1:313254/1‑150 (MQ=255) gggAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGc > 2:34652/1‑148 (MQ=255) gggAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGc < 1:34652/148‑1 (MQ=255) ggAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGCCaa > 2:683225/1‑150 (MQ=255) gAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGTGCTGATCTGCCTGACCTTGACCGACGATCACTGCCa > 2:144856/1‑110 (MQ=255) gAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGTGCTGATCTGCCTGACCTTGACCGACGATCACTGCCa < 1:144856/110‑1 (MQ=255) gCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGCCGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGCCAACGGTAGCgtgt > 1:38436/1‑150 (MQ=255) | TATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCACCATGGCTTTCAACACCATCGAGGAACTTCTCGAGGACTACCGGCAAGGCAAGATGGTCCTGCTGGTGGATGACGAGGACCGGGAAAACGAGGGCGATCTGCTGATCGCCGCCGAGCGCTGCGACGCCCAGGCCATCAACTTCATGGCTCGCGAGGCGCGCGGGCTGATCTGCCTGACCTTGACCGACGATCACTGCCAGCGCCTGGGCCTGGAGCAGATGGTTCCGGCCAACGGTAGCGTGT > NC_002947/4338891‑4339178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |