| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,353,340 | C→G | 29.6% | P141R (CCG→CGG) | yrpB → | 2‑nitropropane dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,353,340 | 0 | C | G | 29.6% | 37.6 / 20.6 | 27 | P141R (CCG→CGG) | yrpB | 2‑nitropropane dioxygenase |
| Reads supporting (aligned to +/- strand): ref base C (9/10); new base G (6/2); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
AAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCGG > NC_002947/4353192‑4353484 | aaGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCg > 2:315406/1‑150 (MQ=255) aGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGc > 1:288362/1‑150 (MQ=255) cAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAg < 1:173354/150‑1 (MQ=255) ggCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTATACGAGCAGAGCTGCCTGCTGGTCCTAGAGTTGCGGTCGCATGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGc > 1:775767/1‑150 (MQ=255) aGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAActgc < 2:106766/150‑1 (MQ=255) attaCAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGc < 1:335800/150‑1 (MQ=255) acggCGAGGGGGGCGCCTTTTTCCCGGCCCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCggg < 2:775767/147‑1 (MQ=255) ggggggggCGCCGATTCCCCGCCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAg < 2:590569/145‑1 (MQ=255) gcCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGt > 2:593983/1‑150 (MQ=255) tttCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCaa < 2:41775/150‑1 (MQ=255) tttCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCaa > 1:41775/1‑150 (MQ=255) ccACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCa < 1:315406/150‑1 (MQ=255) ttCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGcc < 1:537814/150‑1 (MQ=255) gaGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCctggctg > 2:81075/1‑150 (MQ=255) cctgcTGGTCGACGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTGCAGCGCCACGACCGTTGAAGAAGCGGCCTGGCTGGAGCGCCATgg > 1:144250/1‑150 (MQ=255) gctgGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCGGCAGGCCGaaa > 2:364573/1‑61 (MQ=255) gctgGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCGGCAGGCCGaaa < 1:364573/62‑2 (MQ=255) ctgGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCtgc < 1:171336/150‑1 (MQ=255) cccGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCAt > 2:121147/1‑150 (MQ=255) gtgAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGaa > 1:452727/1‑150 (MQ=255) agttCGGCCTGCGGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGcc > 2:127418/3‑150 (MQ=255) agttCGGCCTGCGGCAGGCCGAAATGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGcc > 2:400455/3‑150 (MQ=255) gttCGGCCTGCGGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCa > 2:717655/2‑150 (MQ=255) ttCGGCCTGCGGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGatcat > 1:529979/1‑122 (MQ=255) ttCGGCCTGCGGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGatcat < 2:529979/122‑1 (MQ=255) ttCGGCCTGCGGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCAt > 1:329920/1‑150 (MQ=255) ccTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCgg < 1:121147/150‑1 (MQ=255) | AAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCGG > NC_002947/4353192‑4353484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |