Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,395,564 T→C 12.9% D85G (GAC→GGC)  PP_3871 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,395,5640TC12.9% 75.5 / 4.0 31D85G (GAC→GGC) PP_3871hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (14/13);  new base C (2/2);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

AGCCAGCCGAGAAGCTGAAGCGGTAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCTTTGTCATCAGCGGTGATCACATAGGCCGAGGGG  >  NC_002947/4395415‑4395704
                                                                                                                                                     |                                                                                                                                            
agccagccGAGAAGCTGAAGCGGTAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCGCCAGGCCGCCATCCGACCAAGTCCCGCCACAACGCCTTGCGAAAGTCGTGCAGCAGGt                                                                                                                                              <  1:989/150‑1 (MQ=255)
       cGAGAAGCTGAAGCGGTAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCggcggc                                                                                                                                       <  2:242109/150‑1 (MQ=255)
             gCTGAAGCGGTAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTCGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCctgctg                                                                                                                                 >  2:516329/1‑150 (MQ=255)
                      gTAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTc                                                                                                                        >  2:324120/1‑150 (MQ=255)
                       tAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGCCGCCGACctgctgca                                                                                                                               >  1:359144/1‑140 (MQ=255)
                       tAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGCCGCCGACctgctgca                                                                                                                               <  2:359144/142‑3 (MQ=255)
                         gACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTc                                                                                                                     <  2:494197/150‑1 (MQ=255)
                             accCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCgcgc                                                                                                                          <  1:17135/141‑1 (MQ=255)
                             accCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCgcgc                                                                                                                          >  2:17135/1‑141 (MQ=255)
                             accCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTg                                                                                                                 <  1:23188/150‑1 (MQ=255)
                             accCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTg                                                                                                                 <  1:789892/150‑1 (MQ=255)
                                     aCGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGCGTTATCTTCGCGACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCAt                                                                                                         >  2:625916/1‑150 (MQ=255)
                                                                   gtAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGAt                                                                           <  1:324120/150‑1 (MQ=255)
                                                                     aGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTc                                                                                                                        <  1:403917/103‑1 (MQ=255)
                                                                     aGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTc                                                                                                                        >  2:403917/1‑103 (MQ=255)
                                                                                tCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACt                                                              >  2:85258/1‑150 (MQ=255)
                                                                                      tcatcaCCAGGCCGCCATCCGACCAAGGCCCGCCACAACGCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAacac                                                                                                <  1:769972/110‑1 (MQ=255)
                                                                                      tcatcaCCAGGCCGCCATCCGACCAACGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAacac                                                                                                >  2:769972/1‑110 (MQ=255)
                                                                                       catcaCCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCt                                                       <  2:586940/150‑1 (MQ=255)
                                                                                        atcaCCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTg                                                      >  2:641828/1‑150 (MQ=255)
                                                                                        atcaCCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGCCGCCGACctgctg                                                                                                                                 >  1:794753/1‑75 (MQ=38)
                                                                                        atcaCCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGCCGCCGACctgctg                                                                                                                                 <  2:794753/75‑1 (MQ=38)
                                                                                         tcaCCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCtac                                                     >  2:140975/1‑148 (MQ=255)
                                                                                             cAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCt                                                 >  1:741816/1‑150 (MQ=255)
                                                                                                             cAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCttt                                 >  1:547107/1‑150 (MQ=255)
                                                                                                             cAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCttt                                 >  1:280025/1‑150 (MQ=255)
                                                                                                                   ccGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCgcgc                                                                                                                          <  1:677430/55‑1 (MQ=255)
                                                                                                                   ccGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCgcgc                                                                                                                          >  2:677430/1‑55 (MQ=255)
                                                                                                                               cTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCTTTGTCATCAGCGGTGATcac               >  2:374776/1‑150 (MQ=255)
                                                                                                                                   cGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAGGCTAGCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCTTTGTCATCAGCGGTGATCACATAg           <  1:96078/150‑1 (MQ=255)
                                                                                                                                   cGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCTTTGTCATCAGCGGTGATCACATAg           <  1:85258/150‑1 (MQ=255)
                                                                                                                                            tGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCCAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCTTTGTCATCAGCGGTGCACACATAGGCCGAgggg  <  1:625916/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
AGCCAGCCGAGAAGCTGAAGCGGTAGACCACCCGCGCACGGTTGATCAGTACCAACCCGCCACCGTCGTAGACGATGGGGTCGTACTCATCACCAGGCCGCCATCCGACCAAGGCCCGCCACAACTCCTTGCGAAAGTCGTGCAGCAGGTCGGCGGCCTGCTGGCCGCGCTCGTCCTTGGTGTCCATGGCGAACACCACCTCAAAGCGATCCTCGATGTCCTGGGTAACTGCGTTCTGCGCCTTGTTCTCGCCGGCTTTGTCATCAGCGGTGATCACATAGGCCGAGGGG  >  NC_002947/4395415‑4395704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑