| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,479,750 | C→A | 11.8% | Q353K (CAA→AAA) | ybdR → | Zn‑dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,479,750 | 0 | C | A | 11.8% | 94.3 / 2.4 | 34 | Q353K (CAA→AAA) | ybdR | Zn‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (14/16); new base A (2/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
AGCGTGCCCGGCGTCTATGCAGGGTTCATTCACGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTA > NC_002947/4479606‑4479895 | aGCGTGCCCGGCGTCTATGCAGGGTTCATTCACGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCg < 1:255610/150‑1 (MQ=255) gCGTGCCCGGCGTCTATGCAGGGTTCATTCACGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGTTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGTGCGCCTGCAACCgg > 2:248665/1‑150 (MQ=255) tGCAGGGTTCATTCACGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCa > 2:113048/1‑150 (MQ=255) aGGGTTCATTCACGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCg > 2:440947/1‑150 (MQ=255) aCGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTag > 2:170442/1‑150 (MQ=255) tCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAgg < 1:603144/150‑1 (MQ=255) tGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTTGAGCACATCGAAGCAGGGAGCCGTCCACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAggctg > 2:146057/1‑148 (MQ=255) ccTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACa > 1:169030/1‑150 (MQ=255) ttCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGaa > 1:791313/1‑99 (MQ=255) ttCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGaa < 2:791313/99‑1 (MQ=255) ttCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAggcgg > 2:527587/1‑136 (MQ=255) ttCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAggcgg < 1:527587/136‑1 (MQ=255) cGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAAt < 1:514366/150‑1 (MQ=255) gACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGAAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATg > 1:82090/1‑150 (MQ=255) gACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGAAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATg > 1:82141/1‑150 (MQ=255) aaaGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCCGACCTGATCGTTATTCACCTCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTc < 2:689839/150‑1 (MQ=255) aGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGa < 1:170442/150‑1 (MQ=255) ggCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGAAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGAt < 2:82141/150‑1 (MQ=255) ggCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGAAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGAt < 2:82090/150‑1 (MQ=255) cTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCa > 2:757028/1‑150 (MQ=255) aTGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAAc < 2:601096/150‑1 (MQ=255) tGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACt < 1:440947/150‑1 (MQ=255) aTGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCa > 1:615136/1‑150 (MQ=255) ttCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGtt > 1:475403/1‑115 (MQ=255) ttCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGtt < 2:475403/115‑1 (MQ=255) tCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCt < 1:248665/150‑1 (MQ=255) aaaaaTACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCg < 1:113048/150‑1 (MQ=255) tACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCa < 1:757028/150‑1 (MQ=255) gAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCa > 1:770073/1‑150 (MQ=255) gAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGt < 2:162187/128‑1 (MQ=255) gAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGt > 1:162187/1‑128 (MQ=255) gcCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAg > 2:384187/1‑98 (MQ=255) gcCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAg < 1:384187/98‑1 (MQ=255) ccTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTa < 2:169030/150‑1 (MQ=255) | AGCGTGCCCGGCGTCTATGCAGGGTTCATTCACGGCTTCATGTTCGGTGATGCCTTCGACAAAGGCCTGACCTTCAAGATGGGTCAGACCCATGTTCAAAAATACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTA > NC_002947/4479606‑4479895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |