| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,528,397 | T→A | 20.0% | intergenic (+28/‑48) | purB → / → PP_4017 | adenylosuccinate lyase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,528,397 | 0 | T | A | 20.0% | 52.6 / 9.4 | 25 | intergenic (+28/‑48) | purB/PP_4017 | adenylosuccinate lyase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/10); new base A (2/3); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01 | |||||||||||
CGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTG > NC_002947/4528254‑4528537 | cgcTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCgg < 1:602483/150‑1 (MQ=255) ccTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTAAAACGCCCGGCCAGGCCGGGCGttgtac < 1:417138/150‑5 (MQ=255) ccTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGttt < 2:399830/134‑1 (MQ=255) ccTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGaaa < 2:530091/150‑1 (MQ=255) ccTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGACTGGCCGGGCGttt > 1:399830/1‑134 (MQ=255) aTGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGt > 1:522435/1‑150 (MQ=255) cAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGttttt > 1:106532/1‑150 (MQ=255) cAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATa < 2:549398/146‑1 (MQ=255) cAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATa > 1:549398/1‑146 (MQ=255) ggCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGaa < 2:106532/150‑1 (MQ=255) gcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGtt > 1:252636/1‑94 (MQ=255) gcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGtt > 1:253389/1‑94 (MQ=255) gcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGtt < 2:253389/94‑1 (MQ=255) gcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGtt < 2:252636/94‑1 (MQ=255) cccccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTAc < 1:182834/111‑1 (MQ=255) cccccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTAc > 2:182834/1‑111 (MQ=255) ccccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACt > 1:514496/1‑150 (MQ=255) tacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTc > 2:579571/1‑150 (MQ=255) cAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGaa > 2:321736/1‑150 (MQ=255) tAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGactac < 2:522435/150‑1 (MQ=255) aGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTg < 1:579571/150‑1 (MQ=255) cTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtttttt > 2:451041/1‑67 (MQ=255) cTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtttttt < 1:451041/67‑1 (MQ=255) aaCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgctgc > 1:676215/1‑150 (MQ=255) cGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGctgctg < 1:486549/150‑1 (MQ=255) | CGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTG > NC_002947/4528254‑4528537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |