| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,528,861 | C→A | 11.4% | N139K (AAC→AAA) | PP_4017 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,528,861 | 0 | C | A | 11.4% | 97.0 / 2.7 | 35 | N139K (AAC→AAA) | PP_4017 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (17/14); new base A (2/2); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
TGGACCCTGCTGGTGCAGGCCGTTGACCAGTTCGTTCCGGAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGTGGACCCTGGAGCCCGGCGA > NC_002947/4528712‑4529010 | tGGACCCTGCTGGTGCAGGCCGTTGACCAGTTCGTTCCGGAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAAc < 1:659898/150‑1 (MQ=255) cAGGCCGTTGACCAGTTCGTTCCGGAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCctg < 2:359605/150‑1 (MQ=255) gCCGTTGACCAGTTCGTTCCGGAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAAATACGATGTGTTCctgctg > 1:756903/1‑150 (MQ=255) cGTTGACCAGTTCGTTCCGGAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCa > 1:558159/1‑150 (MQ=255) ggAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCaa > 2:421594/1‑150 (MQ=255) ctgGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAg > 1:250039/1‑150 (MQ=255) gAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCCTCGCCGCCCCGGGTGGCAGCGTCGGCGCGGACTTCGACAACTACCAGGTGCTCCTGCTCCAAGGCCACGGCCAGCGCAACTGGAAGGTCGACCAGAtg > 2:154070/1‑150 (MQ=255) ttCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCctgc < 1:631836/103‑1 (MQ=255) ttCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTgcagc > 1:504240/1‑150 (MQ=255) ttCCGCTTCCTGCACAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCctgc > 2:631836/1‑103 (MQ=255) ccGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACCGCCAGCGCAACTGGAAGATCGGCCAGATGTGcagcag < 1:646386/150‑1 (MQ=255) tCCTGCCCAGCTGGAGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACa < 1:281596/150‑1 (MQ=255) cTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGt < 1:421594/150‑1 (MQ=255) tGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCg < 2:497907/126‑1 (MQ=255) tGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCg > 1:497907/1‑126 (MQ=255) tGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTc > 1:777119/1‑150 (MQ=255) cccAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCg > 2:414507/1‑150 (MQ=255) cccAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAAATACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATc > 1:297949/1‑123 (MQ=255) cccAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAAATACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATc < 2:297949/123‑1 (MQ=255) gCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGa < 2:282785/150‑1 (MQ=255) gATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGcc > 2:51047/1‑150 (MQ=255) gATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGcc > 2:50970/1‑150 (MQ=255) cGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCt < 2:250039/150‑1 (MQ=255) gTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTg > 2:593304/1‑150 (MQ=255) gTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTg > 2:277124/1‑150 (MQ=255) tgatgaTCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAAATACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGc < 2:756903/150‑1 (MQ=255) gatCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTAc > 1:445819/1‑52 (MQ=255) gatCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTAc < 2:445819/52‑1 (MQ=255) gCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTgg > 1:390500/1‑150 (MQ=255) gTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAg < 1:453139/150‑1 (MQ=255) ccGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGATCAGAGCGGCGAGt > 2:130218/1‑145 (MQ=255) ccGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGt < 1:130218/145‑1 (MQ=255) cACTTCGACAACTACTATGTGTTTCTGCTGTAAGGCCACGGCCAGCGCAACTTGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTCGAGCACTTTGACCAGCGGTTTGTGGCGGGCTTCGTTCATAGCGGCGAGTGGACCCTg < 1:100112/150‑1 (MQ=255) aCTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGctgctg < 1:790964/92‑1 (MQ=255) aCTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGctgctg > 2:790964/1‑92 (MQ=255) cTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGTGGACCCTGGa < 1:414507/150‑1 (MQ=255) cTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGTGGACCCTGGAGCCCGGCGa < 1:51047/150‑1 (MQ=255) cTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGTGGACCCTGGAGCCCGGCGa < 1:50970/150‑1 (MQ=255) cTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGTGGACCCTGGAGCCCGGCGa > 2:745118/1‑150 (MQ=255) | TGGACCCTGCTGGTGCAGGCCGTTGACCAGTTCGTTCCGGAAGTGGCCGAACTGCTGGAAAACTTCCGCTTCCTGCCCAGCTGGCGTATCGATGACGTGATGATCAGCTTCGCCGCCCCGGGTGGCAGCGTCGGCCCGCACTTCGACAACTACGATGTGTTCCTGCTGCAAGGCCACGGCCAGCGCAACTGGAAGATCGGCCAGATGTGCAGCAGTGACAGTCCGCTGCTGGAGCACGCCGACCTGCGTATCCTGGCCGAGTTCGAGCAGAGCGGCGAGTGGACCCTGGAGCCCGGCGA > NC_002947/4528712‑4529010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |