| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 210,614 | A→G | 14.7% | T5374A (ACC→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 210,614 | 0 | A | G | 14.7% | 73.7 / 6.4 | 34 | T5374A (ACC→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (15/14); new base G (3/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01 | |||||||||||
ACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCG > NC_002947/210468‑210760 | aCGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGATGGTGACCTTGACCAGGTCGCCt > 2:501937‑M2/1‑149 (MQ=255) cGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGt < 1:744662/150‑1 (MQ=255) ggCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAAc < 1:230446/150‑1 (MQ=255) gCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACg > 2:255515/1‑150 (MQ=255) ggACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAAcc > 1:731051/1‑150 (MQ=255) aagtGACGCTGGACAAGACGCCGGTCAGAACGTCGGTCACCGACGAGCCAGGTAACCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATGACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCa > 2:98753/4‑150 (MQ=255) aCAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGTGACCTTGACCAGGTCGCCttcgttgcctggggtacct < 1:234571‑M2/96‑20 (MQ=255) aCAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGTGACCTTGACCAGGTCGCCttcgttgcctggggtacct > 2:234571‑M2/1‑77 (MQ=255) aGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTc > 2:59706/1‑150 (MQ=39) gTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACC > 1:40808‑M2/1‑66 (MQ=255) gTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACC < 2:40808‑M2/66‑1 (MQ=255) tggctcGTCGGTGACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGt > 2:427880/6‑150 (MQ=255) aGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCCCTGGCTCACGACCTGGTc > 2:514389/1‑150 (MQ=25) gCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCa > 1:610986/1‑111 (MQ=37) gCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCa < 2:610986/111‑1 (MQ=38) gCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCg > 2:259616/1‑150 (MQ=35) cGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGa < 2:731051/150‑1 (MQ=35) gTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACC < 1:623114‑M2/58‑1 (MQ=255) gTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACC > 2:623114‑M2/1‑58 (MQ=255) tgaCCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGa < 1:427880/148‑1 (MQ=25) cAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGccc < 1:59706/150‑1 (MQ=38) cccAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCAc < 1:391785/150‑1 (MQ=35) ccAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGATCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCAcc > 1:245976/1‑150 (MQ=25) gCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg > 1:705154/1‑140 (MQ=32) gCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg < 2:705154/140‑1 (MQ=33) aaCGAAGGCGACCTGGTCAAGGTCACCttcgttgcctggggtacccggctcgtcggtgaccgacgtgctgaccggggttttgtcgagt > 2:370473‑M2/1‑27 (MQ=255) aaCGAAGGCGACCTGGTCAAGGTCACCttcgttgcctggggtacccggctcgtcggtgaccgacgtgctgaccggggttttgtcgagt < 1:370473‑M2/88‑62 (MQ=255) gAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGcc > 2:683545/1‑150 (MQ=32) ggCGACCTGGTCAAGGTCGCCttcgttgcctggggtacccggctcgtcggtgaccgacgtgctgaccggggttttgtc < 2:166015‑M2/78‑58 (MQ=255) ggCGACCTGGTCAAGGTCGCCttcgttgcctggggtacccggctcgtcggtgaccgacgtgctgaccggggttttgtc > 1:166015‑M2/1‑21 (MQ=255) cGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCga < 1:259616/150‑1 (MQ=32) gTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGACCGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGATCAACAATGCCCAg > 1:646916/1‑117 (MQ=16) gTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg < 2:646916/117‑1 (MQ=33) gTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCg < 1:255515/150‑1 (MQ=33) | ACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCG > NC_002947/210468‑210760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |