| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,719,068 | C→T | 11.8% | S258N (AGT→AAT) | PP_4176 ← | 5‑oxo‑L‑prolinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,719,068 | 0 | C | T | 11.8% | 84.8 / 3.9 | 34 | S258N (AGT→AAT) | PP_4176 | 5‑oxo‑L‑prolinase |
| Reads supporting (aligned to +/- strand): ref base C (16/14); new base T (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
GCTTGAACCCGTGCATCTGCTCCCCCAGGCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATGAATCGCGTATGCGGGTGA > NC_002947/4718919‑4719211 | gCTTGAACCCGTGCATCTGCTCCCCCAGGCTGCCAAGCCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCacac > 1:661803/1‑150 (MQ=255) aaCCCGTGCATCTGCTCCCCCAGGCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAATCGCTCCGGGTACTTCTCGCCCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCAc < 2:451694/150‑1 (MQ=255) cTGCTCCCCCAGGCTGCCAAACCGCCCCACCACATCCGACCCCAACATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGAc < 1:272597/150‑1 (MQ=255) tCCCCCAGGCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATAc > 1:141356/1‑150 (MQ=255) ccccAGGCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAg > 2:160490/1‑150 (MQ=255) ccccAGGCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGCTGCAGCACACTCCACGACAAATCGACATACAg > 2:169665/1‑150 (MQ=255) ggCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCg < 2:614647/150‑1 (MQ=255) cTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCggg < 2:293934/150‑1 (MQ=255) gCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTa < 1:160490/150‑1 (MQ=255) ccAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAg < 2:217520/150‑1 (MQ=255) cGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGtcctcc > 2:615258/1‑150 (MQ=255) gCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCca > 1:711700/1‑150 (MQ=255) ccacATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACATTCCa < 1:24926/109‑1 (MQ=255) ccacATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACATTCCa < 1:24757/109‑1 (MQ=255) ccacATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACATTCCa > 2:24926/1‑109 (MQ=255) ccacATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACATTCCa > 2:24757/1‑109 (MQ=255) ccacATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGCTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATc < 1:169665/150‑1 (MQ=255) acaTCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATcgc < 2:404073/150‑1 (MQ=255) aaCCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGa > 1:448140/1‑150 (MQ=255) aCCGCTCCGGGTACTTCTCGACCAGCGCCAGCAATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGaa > 2:96268/1‑150 (MQ=255) ttCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTc > 2:355238/1‑150 (MQ=255) tcGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCgg < 1:752486/125‑1 (MQ=255) tcGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCgg > 2:752486/1‑125 (MQ=255) gACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCtggtg > 2:51187/1‑150 (MQ=255) aGCGCCAGCAATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCgg < 1:96268/150‑1 (MQ=255) ccagccaTTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGc < 2:599756/134‑1 (MQ=255) ccagccaTTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGc > 1:599756/1‑134 (MQ=255) ttGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGGATCTCCAtgctg > 1:533676/1‑150 (MQ=255) aTCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGAt > 2:394099/1‑150 (MQ=255) aTCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGAt < 1:615258/150‑1 (MQ=255) aTCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGAt < 2:711700/150‑1 (MQ=255) tCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATg > 2:197475/1‑150 (MQ=255) tCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATg > 1:380732/1‑150 (MQ=255) gCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATGAATc > 2:91945/1‑150 (MQ=255) aaaTACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATGAATcgc < 2:380732/150‑1 (MQ=255) agaacacTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATGAATCGCGTATGGGGGTGa > 2:153433/4‑150 (MQ=255) | GCTTGAACCCGTGCATCTGCTCCCCCAGGCTGCCAAACCGCCCCACCACATCCGACCCCAGCATGAACCGCTCCGGGTACTTCTCGACCAGCGCCAGCCATTCCTTGCGCGGCGCGCCCTGCTCATCCAGCAAATACGGTTGCAGCACACTCCACGACAAATCGACATACAGGTTCGGGTAGTCCTCCAACAATCGCGTCAGCACCGGCAGTAGAAAGTCCATCTGCTTCTGGTGGCGGTGTATCTCCATGCTGCTGCCCGCGTGCGCCCAGATGAATCGCGTATGCGGGTGA > NC_002947/4718919‑4719211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |