Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,727,359 T→C 15.5% A23A (GCA→GCG)  brnQ ← branched‑chain amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,727,3590TC15.5% 56.0 / 2.1 25A23A (GCA→GCG) brnQbranched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (11/10);  new base C (2/2);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAGTGCATCCATGCCGCCACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGATTTCCCTTTGGGTAAGGGGAA  >  NC_002947/4727210‑4727491
                                                                                                                                                     |                                                                                                                                    
ctCAGTGCATCCATGCCGCCACCGACTGTGGACAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCt                                                                                                                                      <  2:300934/150‑1 (MQ=255)
    gTGCATCCATGCCGCCACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCAc                                                                                                                                  >  1:361927/1‑150 (MQ=255)
    gTGCATCCATGCCGCCACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCAc                                                                                                                                  >  1:390891/1‑150 (MQ=255)
        aTCCATGCCGCCACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCAc                                                                                                                              >  2:502771/1‑150 (MQ=255)
            aTGCCGCCACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAAGCGGGAAGATGATATTGCCCGCAGCCCCGaaa                                                                                                                          >  2:187234/1‑150 (MQ=255)
                cgccACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAgcg                                                                                                                      <  2:390891/150‑1 (MQ=255)
                  ccACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGACCGACGATAGGCGGGAAGATGAAATTGCCTGGACCCACGAAACGCGCa                                                                                                                    >  2:359266/1‑150 (MQ=255)
                        aCCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTc                                                                                                              <  2:361927/150‑1 (MQ=255)
                         ccTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCa                                                                                                             >  2:214573/1‑150 (MQ=255)
                           ttcgCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCCGCACCCACGAAAAGCGCAAACGTCATa                                                                                                           >  2:198807/4‑150 (MQ=255)
                                                                    aGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCAt                                                                  <  1:384787/150‑1 (MQ=255)
                                                                     gTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCCGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCAtt                                                                 <  1:198807/150‑1 (MQ=255)
                                                                                  ccATGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACca                                                    >  2:134731/1‑150 (MQ=255)
                                                                                        ccgccATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTCCATTTAAGGTAGTACCACActgc                                              <  1:359266/150‑1 (MQ=255)
                                                                                         cgccATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCAt                                                                  >  1:799010/1‑129 (MQ=255)
                                                                                         cgccATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCAt                                                                  <  2:799010/129‑1 (MQ=255)
                                                                                         cgccATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACActgct                                             <  2:284944/150‑1 (MQ=255)
                                                                                                 atACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCgg                                     <  1:502771/150‑1 (MQ=255)
                                                                                                           gCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATc                                                                                       >  1:722758/1‑90 (MQ=255)
                                                                                                           gCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATc                                                                                       <  2:722758/90‑1 (MQ=255)
                                                                                                           gCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGagag                           >  2:628397/1‑150 (MQ=255)
                                                                                                               gACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCCGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGa                       <  1:187234/150‑1 (MQ=255)
                                                                                                                     aGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGATTTccc                 >  2:280907/1‑150 (MQ=255)
                                                                                                                       gCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGATTTCCCtt               <  1:214573/150‑1 (MQ=255)
                                                                                                                          gacgaTAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGATTTCCCTTTgg            <  2:560772/150‑1 (MQ=255)
                                                                                                                                    gggAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGATTTCCCTTTGGGTAAGGGGaa  >  1:687188/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                    
CTCAGTGCATCCATGCCGCCACCGACCTTGGCCAGGGCGACCACCGTGATGACCGGCAGGCCCACGGCAGTGACCAGGAAGCCCAGGGCCGCCATCCATACGTGCGGGCCAGACTGCAGGCCGACGATAGGCGGGAAGATGATATTGCCTGCACCCACGAAAAGCGCAAACGTCATAAAGCCAAGCGCCAGGATATCCTGGCCTTTGAGAACTTTCATTTAAGGTAGTACCACACTGCTGAAATCGGGATTCGAGAGGGGATTTCCCTTTGGGTAAGGGGAA  >  NC_002947/4727210‑4727491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑