Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,792 T→C 9.1% S27G (AGC→GGC)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,7920TC 9.1% 125.0 / 2.6 44S27G (AGC→GGC) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (20/20);  new base C (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTCGCT  >  NC_002947/4744645‑4744938
                                                                                                                                                   |                                                                                                                                                  
aaCCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACtgctgc                                                                                                                                                  <  1:780306/150‑1 (MQ=255)
 aCCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTgcg                                                                                                                                                 <  2:385276/150‑1 (MQ=255)
          gATGTAACTGGCCAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                        >  2:593053/1‑150 (MQ=255)
            tGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgcgc                                                                                                                                      >  1:462293/1‑150 (MQ=255)
                aCTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAAcc                                                                                                                                  >  2:23283/1‑150 (MQ=255)
                 cTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCt                                                                                                                                 >  2:96413/1‑150 (MQ=255)
                             tCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGcc                                                                                                                     >  1:184287/1‑150 (MQ=255)
                                  gcgAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAg                                                                                                                >  2:694561/1‑150 (MQ=255)
                                  gcgAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAg                                                                                                                >  1:169650/1‑150 (MQ=255)
                                        cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGc                                                                                                                      >  1:512106/1‑138 (MQ=255)
                                        cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGc                                                                                                                      <  2:512106/138‑1 (MQ=255)
                                        cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatgag                                                                                                                                        >  1:408514/1‑114 (MQ=255)
                                        cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatgag                                                                                                                                        <  2:408514/120‑7 (MQ=255)
                                          gCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCa                                                                                                        >  2:392464/1‑150 (MQ=255)
                                           cGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCaa                                                                                                       <  1:299421/150‑1 (MQ=255)
                                             aCTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATg                                                                                                     >  1:334602/1‑150 (MQ=255)
                                                  cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATGGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg                                                                                                                                              >  1:762796/1‑104 (MQ=255)
                                                  cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg                                                                                                                                              <  2:762796/104‑1 (MQ=255)
                                                        cgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGc                                                                                                    <  2:55827/140‑1 (MQ=255)
                                                        cgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGc                                                                                                    >  1:55827/1‑140 (MQ=255)
                                                          aaTCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGc                                                                                              >  2:206017/1‑144 (MQ=255)
                                                          aaTCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGc                                                                                              <  1:206017/144‑1 (MQ=255)
                                                          aaTCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCaa                                                                                        >  1:544604/1‑150 (MQ=255)
                                                            tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                                      <  2:334602/150‑1 (MQ=255)
                                                                         cgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGAt                                                                         <  2:544604/150‑1 (MQ=255)
                                                                           cgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTTACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCa                                                                       <  1:96413/150‑1 (MQ=255)
                                                                                   agcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCa                                                               <  1:742000/150‑1 (MQ=255)
                                                                                    gcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCCTCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                              <  1:485762/150‑1 (MQ=255)
                                                                                    gcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                              <  1:593053/150‑1 (MQ=255)
                                                                                               tcatcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAgcgc                                                                                                             >  2:161650/1‑92 (MQ=255)
                                                                                               tcatcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAgcgc                                                                                                             <  1:161650/92‑1 (MQ=255)
                                                                                               tcatcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCa                                                                                             >  2:267916/1‑108 (MQ=255)
                                                                                               tcatcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCa                                                                                             <  1:267916/108‑1 (MQ=255)
                                                                                                           cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                      <  2:397143/135‑1 (MQ=255)
                                                                                                           cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                      >  1:397143/1‑135 (MQ=255)
                                                                                                           cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                       <  1:694561/150‑1 (MQ=255)
                                                                                                              tGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGc                                                                   <  1:581013/119‑1 (MQ=255)
                                                                                                              tGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGc                                                                   >  2:581013/1‑119 (MQ=255)
                                                                                                               ggAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGATGCACCTCCAGTTGCTTGCGCAAGCGCTTGAc                                   <  2:184287/150‑1 (MQ=255)
                                                                                                                     cAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGa                             >  2:457769/1‑150 (MQ=255)
                                                                                                                        cGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTg                          <  2:169650/150‑1 (MQ=255)
                                                                                                                          gTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgca                        <  1:23283/150‑1 (MQ=255)
                                                                                                                           tGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCCGCCACGTCAGTGCAGCCAAAGGCGTACGACAGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgcag                       <  2:462293/150‑1 (MQ=255)
                                                                                                                               cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAcc                   >  1:11926/1‑150 (MQ=255)
                                                                                                                                         gcgGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAgctgct         >  2:679306/1‑150 (MQ=255)
                                                                                                                                                tgctgcGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTgctcttcgct  <  2:750750/150‑1 (MQ=255)
                                                                                                                                                tgctgcGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTgctcttcgct  >  1:202814/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTCGCT  >  NC_002947/4744645‑4744938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑