| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,767,864 | A→G | 13.5% | intergenic (+33/+36) | fpvA → / ← PP_4218 | TonB‑dependent outer membrane ferripyoverdine receptor FpvA/lipase/esterase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,767,864 | 0 | A | G | 13.5% | 85.2 / 6.2 | 37 | intergenic (+33/+36) | fpvA/PP_4218 | TonB‑dependent outer membrane ferripyoverdine receptor FpvA/lipase/esterase family protein |
| Reads supporting (aligned to +/- strand): ref base A (16/16); new base G (2/3); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 | |||||||||||
TTCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCACC > NC_002947/4767724‑4768005 | ttCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtt < 1:79800/150‑1 (MQ=255) gACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGttttt > 1:420956/1‑150 (MQ=255) tacCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCt < 2:52248/150‑1 (MQ=255) cAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGaa > 2:740311/1‑150 (MQ=255) aGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGaaa > 2:787502/1‑150 (MQ=255) aGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGt > 1:189821/1‑114 (MQ=255) aGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGt < 2:189821/114‑1 (MQ=255) aacTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgc < 2:574069/150‑1 (MQ=255) ccTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgct < 1:740311/150‑1 (MQ=255) gAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttttt < 2:758314/110‑1 (MQ=255) gAGCCCGTACGACGTGTATGGTGACCAACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttttt > 1:758314/1‑110 (MQ=39) cccGTACGACGTGTATGGTGACCCACGNAACTTCACCATCNCCGCCGNGNAGAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGc > 1:416111/1‑150 (MQ=255) gTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGc < 2:324983/96‑1 (MQ=255) gTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGc > 1:324983/1‑96 (MQ=255) gacgTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGacc < 2:420956/150‑1 (MQ=255) cgTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGaccac < 1:787502/150‑1 (MQ=255) gtATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTc < 1:722482/141‑1 (MQ=255) gtATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTc > 2:722482/1‑141 (MQ=255) ccACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACCGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCt < 2:93964/150‑1 (MQ=255) aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGc < 2:497785/146‑1 (MQ=255) aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGc > 1:497785/1‑146 (MQ=255) aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATACCTGAACGGCTCCCTTACAGATGCTGCTGCAGCCCTGCTACCACCATCCCCCGGCCTTc > 2:185559/1‑150 (MQ=255) aaCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGc > 2:569840/1‑142 (MQ=255) aaCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGc < 1:569840/142‑1 (MQ=255) ttCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttt > 2:379140/1‑74 (MQ=38) ttCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttt < 1:379140/74‑1 (MQ=255) ttCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGaaa < 2:192823/91‑1 (MQ=255) ttCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGaaa > 1:192823/1‑91 (MQ=255) ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGAGGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCgatgagcc > 1:598949/1‑147 (MQ=255) aaGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGaa < 2:416111/150‑1 (MQ=255) gATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGa > 2:671232/1‑150 (MQ=255) gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATgg < 2:524511/150‑1 (MQ=255) cGTTGCACGCAAACGCCCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTCCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGt < 1:185559/150‑1 (MQ=255) aaaaaCCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgc < 1:162445/62‑1 (MQ=255) aaaaaCCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgc > 2:162445/1‑62 (MQ=255) aataCCGCACCCAGAAGCGGTATTGGCGTTGATGCCTGACACGCACCCTTAAAGATGATGCTGCAGTGCCTCGACCACCATAGCCAGGCCTTCATGTGCCTCCTCAATGATTGAACCCATACGCATGACGTCATGGGTCACTCCCGGGAt > 2:70634/4‑150 (MQ=255) ccGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCAcc > 1:755326/1‑150 (MQ=255) | TTCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCACC > NC_002947/4767724‑4768005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |