Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,866,075 T→A 16.7% D169V (GAC→GTC)  PP_4277 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,866,0750TA16.7% 70.3 / 8.6 30D169V (GAC→GTC) PP_4277GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (12/13);  new base A (2/3);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

CCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGT  >  NC_002947/4865932‑4866207
                                                                                                                                               |                                                                                                                                    
ccAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGTCGCTGGACCAGCg                                                                                                                                >  1:753891/1‑150 (MQ=255)
    tGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGTCGCTGGACCAGCGCCAg                                                                                                                            <  2:753891/150‑1 (MQ=255)
    tGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCCATCATGTTGGCCTGTCGCTGGACCAGCGCCAg                                                                                                                            <  1:104521/150‑1 (MQ=255)
         ccAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGTACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAAt                                                                                                                       <  1:634749/150‑1 (MQ=255)
           aGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAg                                                                                                                     <  2:604657/150‑1 (MQ=255)
                                    cATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTc                                                                                                                                     <  1:722562/109‑1 (MQ=255)
                                    cATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTc                                                                                                                                     >  2:722562/1‑109 (MQ=255)
                                       aGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGTTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGtttt                                                                                         >  1:111872/1‑150 (MQ=255)
                                                      cgcgAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCa                                                                          <  1:526437/150‑1 (MQ=255)
                                                                       tCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTc                                                                               <  1:490175/128‑1 (MQ=255)
                                                                       tCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTc                                                                               >  2:490175/1‑128 (MQ=255)
                                                                            cgAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACgg                                                    <  1:358692/150‑1 (MQ=255)
                                                                                           gAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCgg                                                                                                    >  1:644440/1‑87 (MQ=255)
                                                                                           gAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCgg                                                                                                    <  2:644440/87‑1 (MQ=255)
                                                                                            aGCAAGCGGGAGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATg                                    >  1:530567/1‑150 (MQ=255)
                                                                                                             gTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCCGGTTTTCCACGGTTTCCTGCAGGAACGGGATGTAGGCGAACGGATAAGTCGGACGCAGGACCGCAAAACTCTCgcc                   >  2:528802/1‑148 (MQ=255)
                                                                                                                       tCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATa                                                 <  1:143424/110‑1 (MQ=255)
                                                                                                                       tCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATa                                                 >  2:143424/1‑110 (MQ=255)
                                                                                                                         gATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCATGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCgg                    >  2:777827/1‑137 (MQ=255)
                                                                                                                         gATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCATGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCgg                    <  1:777827/137‑1 (MQ=255)
                                                                                                                         gATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCtt       >  2:436095/1‑150 (MQ=255)
                                                                                                                           tCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCCTGACCGCAAAACTGTCGGCGACGAAGGTCTTgt     <  2:428525/150‑2 (MQ=255)
                                                                                                                           tCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGa     <  1:528802/150‑1 (MQ=255)
                                                                                                                              tGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGt  >  2:354961/1‑150 (MQ=255)
                                                                                                                                  ggCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTc                      <  2:663230/126‑1 (MQ=255)
                                                                                                                                  ggCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTc                      >  1:663230/1‑126 (MQ=255)
                                                                                                                                     cTGTCGCTGGACCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGa     >  1:77716/1‑140 (MQ=255)
                                                                                                                                     cTGTCGCTGGACCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGa     <  2:77716/140‑1 (MQ=255)
                                                                                                                                     cTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCCCGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGa                                                   >  2:154356/1‑94 (MQ=255)
                                                                                                                                     cTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGa                                                   <  1:154356/94‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                    
CCAGTGCCGCCAGCACCAGTTCGCAGCTGCGCTGGCCATAGGCGGTGAGCACCTCGCGAATGCGCGGCAGGTCACGCGAGACCACGGCTTCGAGCAAGCGGGCGAACAGGTCGAGATAGTCGATCATGTTGGCCTGGCGCTGGTCCAGCGACAGGTAATAGGCACGGCTCATGGCCGGCTGCAGGTTTTCCACGGTTTCCTGCAGGAACGGGTTGTCGGCGAACGGATAGGCCGCACGCATGACCGCAAAACTGTCGGCGACGAAGGTCTTGATGT  >  NC_002947/4865932‑4866207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑