Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,976,257 C→G 8.4% D187H (GAC→CAC)  flgJ ← peptidoglycan hydrolase FlgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,976,2570CG 8.4% 147.8 / 5.3 49D187H (GAC→CAC) flgJpeptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base C (17/27);  new base G (2/2);  total (19/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

TTGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCA  >  NC_002947/4976119‑4976402
                                                                                                                                          |                                                                                                                                                 
ttGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGggcaggca                                                                                                                                        >  2:435900/1‑150 (MQ=255)
   gcagcaTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACTGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCGTGACGgtcaggcagta                                                                                                                                     <  1:504929/150‑4 (MQ=255)
       caTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACggcggc                                                                                                                                 <  1:435900/150‑1 (MQ=255)
            tggCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTAt                                                                                                                            >  2:470463/1‑150 (MQ=255)
             ggCGCCGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCATGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTAtt                                                                                                                           <  2:262882/150‑1 (MQ=255)
                 acgaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCa                                                                                                                       >  1:459992/1‑150 (MQ=255)
                   gaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGt                                                                                                                     >  1:501220/1‑150 (MQ=255)
                   gaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGt                                                                                                                     >  1:725346/1‑150 (MQ=255)
                       gCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTcc                                                                                                                 >  1:410218/1‑150 (MQ=255)
                         gTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACg                                                                                                                                                >  1:372824/1‑117 (MQ=255)
                         gTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACg                                                                                                                                                <  2:372824/117‑1 (MQ=255)
                            gCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGa                                                                                                            <  1:470463/150‑1 (MQ=255)
                              tGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACg                                                                                                          >  1:607891/1‑150 (MQ=255)
                                tCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGc                                                                                                        >  1:519616/1‑150 (MQ=255)
                                 cGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCa                                                                                                       <  2:305595/150‑1 (MQ=255)
                                  ggTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCAc                                                                                                      <  1:274946/150‑1 (MQ=255)
                                         gCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGCCAGCCACGGCGGCTTTATTGGCAGTTTCCGCCGACGGCACTATACCg                                                                                               <  1:550421/150‑1 (MQ=255)
                                          ccTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCgg                                                                                              >  2:343789/1‑150 (MQ=255)
                                          ccTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCgg                                                                                              >  2:25783/1‑150 (MQ=255)
                                           cTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGc                                                                                             <  2:725346/150‑1 (MQ=255)
                                           cTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGc                                                                                             <  2:725180/150‑1 (MQ=255)
                                                   gggcCAGCGGGGGCTGGGCCACGGCACGGCCGACAATGCGCGGGCCGGTTTCCTGCACGGCGAAGGCATTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGc                                                                                     <  2:336012/148‑1 (MQ=255)
                                                       cAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCCGTTTCCGCCGACGGCACGATACCGGCCAACAGGcggtc                                                                                 >  2:2557/1‑150 (MQ=255)
                                                        aGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGcggtcg                                                                                <  2:696997/150‑1 (MQ=255)
                                                            gTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGa                                                                            <  1:25783/150‑1 (MQ=255)
                                                              ggCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGt                                                                          <  1:343789/150‑1 (MQ=255)
                                                              ggCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGt                                                                          <  2:23819/150‑1 (MQ=255)
                                                              ggCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCCGTCCGATATGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGt                                                                          <  1:494925/150‑1 (MQ=255)
                                                                           cacggcCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAggg                                                             <  2:278831/150‑1 (MQ=255)
                                                                              ggcCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCa                                                          >  1:637283/1‑150 (MQ=255)
                                                                               gcCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGa                                                                            >  2:325419/1‑131 (MQ=255)
                                                                               gcCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGa                                                                            <  1:325419/131‑1 (MQ=255)
                                                                                 cGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGgc                                                       >  2:360923/1‑150 (MQ=255)
                                                                                          gcAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAAc                                                                                         >  1:357536/1‑107 (MQ=255)
                                                                                          gcAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAAc                                                                                         <  2:357536/107‑1 (MQ=255)
                                                                                           cAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGtt                                             <  1:125334/150‑1 (MQ=255)
                                                                                           cAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGtt                                             <  2:501220/150‑1 (MQ=255)
                                                                                                       cGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAAc                                 <  2:381845/150‑1 (MQ=255)
                                                                                                                   ggCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGt                     <  2:601515/150‑1 (MQ=255)
                                                                                                                     ccTTGGCTACTGCTTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCGGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAg                   <  2:145856/150‑1 (MQ=255)
                                                                                                                       ttGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGtt                                             <  1:718577/122‑1 (MQ=255)
                                                                                                                       ttGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGtt                                             >  2:718577/1‑122 (MQ=255)
                                                                                                                            tACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGa            <  2:607891/150‑1 (MQ=255)
                                                                                                                             aCTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAg           <  1:2557/150‑1 (MQ=255)
                                                                                                                              gtgcctgccCGTGACGGTCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAgg          >  1:747000/2‑150 (MQ=255)
                                                                                                                              cTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAgg          <  1:486515/150‑1 (MQ=255)
                                                                                                                                 ccTGCCCGTGACGGTCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTgcgc                       <  2:568520/134‑1 (MQ=255)
                                                                                                                                 ccTGCCCGTGACGGTCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTgcgc                       >  1:568520/1‑134 (MQ=255)
                                                                                                                                      ccGTCACGGGCAGGCACGGCGCCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCCTGGCa  <  2:410218/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
TTGGCAGCATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCA  >  NC_002947/4976119‑4976402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑