| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,025,416 | C→T | 13.8% | P397L (CCA→CTA) ‡ | PP_4429 → | GntR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,025,416 | 0 | C | T | 13.8% | 65.9 / 4.3 | 29 | P397L (CCA→CTA) ‡ | PP_4429 | GntR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (13/12); new base T (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01 | |||||||||||
GCACGGTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAA > NC_002947/5025268‑5025556 | gCACGGTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCa < 2:79822/150‑1 (MQ=255)gCACGGTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCa > 1:79822/1‑150 (MQ=255) gggCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCTAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGcc < 1:400754/150‑1 (MQ=255) gCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCa > 1:19438/1‑150 (MQ=255) cGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCat > 2:310414/1‑150 (MQ=255) gCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGc < 2:646413/150‑1 (MQ=255) agagACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACACTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTAc < 2:302035/150‑1 (MQ=255) acGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGa > 2:543432/1‑96 (MQ=255) acGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGa < 1:543432/96‑1 (MQ=255) ccAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTACTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTa < 1:393854/118‑1 (MQ=255) ccAGAAGCTGGTAGAAGACGAGATGGGGCGCTACCTCTACTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTa > 2:393854/1‑118 (MQ=255) gTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCAcc > 1:745115/1‑150 (MQ=255) aCGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc > 2:202124/1‑82 (MQ=255) aCGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc < 1:202124/82‑1 (MQ=255) gagaTGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCggggg < 2:745115/150‑1 (MQ=255) ccATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCacac < 2:297840/74‑1 (MQ=255) ccATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCacac > 1:297840/1‑74 (MQ=255) cccTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCCGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCggggg > 1:558076/1‑121 (MQ=255) cccTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCCGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCggggg < 2:558076/121‑1 (MQ=255) tCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCAt > 1:299918/1‑150 (MQ=255) ttCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCgg > 2:471711/1‑150 (MQ=255) aaaCGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCggggg < 1:633895/109‑1 (MQ=255) aaaCGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCggggg > 2:633895/1‑109 (MQ=255) ggCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATCTTGCGGTTCCGTCACCGGGGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGAATCAGGCTTTGCATCGGCGCTGAAACAAGTg > 2:304402/1‑150 (MQ=255) tGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTATACGGGTGTG‑CCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGaaa < 2:195733/150‑1 (MQ=255) ccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGa > 1:597101/3‑139 (MQ=255) ccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGa < 2:597101/137‑1 (MQ=255) caaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGgcg < 1:534523/133‑1 (MQ=255) caaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGgcg > 2:534523/2‑134 (MQ=255) | GCACGGTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGT‑TCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAA > NC_002947/5025268‑5025556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |