Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,115,762 C→A 13.4% R24R (CGG→CGT)  PP_4500 ← TrpH family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,115,7620CA13.4% 69.9 / 4.0 30R24R (CGG→CGT) PP_4500TrpH family protein
Reads supporting (aligned to +/- strand):  ref base C (11/15);  new base A (2/2);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

ATGGTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGC  >  NC_002947/5115616‑5115896
                                                                                                                                                  |                                                                                                                                      
atggTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCgg                                                                                                                                     <  2:718174/150‑1 (MQ=255)
     cgcgCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAAc                                                                                                                                <  1:168510/150‑1 (MQ=255)
     cgcgCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAAc                                                                                                                                <  2:154459/150‑1 (MQ=255)
       cgccgcCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGATGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCa                                                                                                                              >  1:774006/1‑150 (MQ=255)
                      aGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGGGCTCATGAGCACggggt                                                                                                                                  <  1:198636/131‑3 (MQ=255)
                      aGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGGGCTCATGAGCACggggt                                                                                                                                  >  2:198636/1‑129 (MQ=255)
                             cTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGcc                                                                                                        >  2:394259/1‑150 (MQ=255)
                                  ccccGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCgg                                                                                                   >  2:773549/1‑150 (MQ=255)
                                      gCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGc                                                                                               <  1:129858/150‑1 (MQ=255)
                                                cgcATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGa                                                                                     <  1:394259/150‑1 (MQ=255)
                                                   aTACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGAGCGTGGACCCCGTGCTCATGAGCCCGGGCAACCATGACCGAAAGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAg                                                                                  >  1:52834/1‑150 (MQ=255)
                                                     aCCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGATGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTg                                                                                <  2:774006/150‑1 (MQ=255)
                                                       ccAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGa                                                                                                  >  1:719014/1‑130 (MQ=255)
                                                       ccAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGa                                                                                                  <  2:719014/130‑1 (MQ=255)
                                                          ggTCAACGCAGGCCTGGCGCGCTTCGCGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGTGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGAt                                                                           <  2:314024/150‑1 (MQ=255)
                                                           gTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCGGCCAGCCCCGGTCAACCAGTACCGAGGGCGCCAGGGCGCCATCGTAGTCGGTGTTGTGACAGTGCAGATc                                                                          >  1:14186/1‑150 (MQ=255)
                                                                                           cTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAgagga                                                           <  2:30878/133‑1 (MQ=255)
                                                                                           cTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAgagga                                                           >  1:30878/1‑133 (MQ=255)
                                                                                           cTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTc                                          >  2:559093/1‑150 (MQ=255)
                                                                                            ttCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCg                                         >  2:537796/1‑150 (MQ=255)
                                                                                                aGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGAtgt                                     <  1:445533/150‑1 (MQ=255)
                                                                                                             cggtcAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGcc                        <  2:701823/150‑1 (MQ=255)
                                                                                                                            cTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTc         <  1:773549/150‑1 (MQ=255)
                                                                                                                               gCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTACAGACCAACATTCCTAAAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTgg      <  2:690027/150‑1 (MQ=255)
                                                                                                                                 aCCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCt    <  2:52834/150‑1 (MQ=255)
                                                                                                                                   cccGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGc  >  1:289759/1‑150 (MQ=255)
                                                                                                                                    ccgggCTCATGAGCACGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGAt                                            <  2:129939/103‑1 (MQ=255)
                                                                                                                                    ccGGGCTCATGAGCACGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGAt                                            >  1:129939/1‑107 (MQ=255)
                                                                                                                                            aTGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCt                                                                                         >  2:464045/1‑54 (MQ=255)
                                                                                                                                            aTGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCt                                                                                         <  1:464045/54‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                      
ATGGTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGC  >  NC_002947/5115616‑5115896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑