Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,168,846 T→C 9.5% V139A (GTG→GCG)  PP_4547 → glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,168,8460TC 9.5% 106.5 / 3.1 42V139A (GTG→GCG) PP_4547glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (19/19);  new base C (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

CCTGGTGCGCCTGCCCTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCTGCGCGAACTGGAGCAGATCGAACCGTTC  >  NC_002947/5168697‑5168991
                                                                                                                                                     |                                                                                                                                                 
ccTGGTGCGCCTGCCCTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGt                                                                                                                                                   >  1:448621/1‑150 (MQ=255)
   ggTGCGCCTGCCCTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGAt                                                                                                                                                >  2:1478/1‑150 (MQ=255)
    gTGCGCCTGCCCTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATg                                                                                                                                               <  2:279968/150‑1 (MQ=255)
        acctgcactgcggcggtgggcacccggcgccagggcaggTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCTAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCt                                                                                                                                           >  2:139528‑M2/40‑150 (MQ=255)
        acctgcactgcggcggtgggcacccggcgccagggcaggTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCAGCTAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCt                                                                                                                                           <  1:139528‑M2/111‑1 (MQ=255)
              ccTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGCGATGGCCTGCGAGc                                                                                                                                     >  2:719076/1‑150 (MQ=255)
              ccTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGCGATGGCCTGCGAGc                                                                                                                                     >  2:719139/1‑150 (MQ=255)
                tGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTg                                                                                                                                   >  1:554703/1‑150 (MQ=255)
                         gTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCAAGCAATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTAc                                                                                                                          <  1:297329/150‑1 (MQ=255)
                               aCCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTc                                                                                                                    >  1:135197/1‑150 (MQ=255)
                                     gcAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACcag                                                                                                              <  1:1478/150‑1 (MQ=255)
                                     gcAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACcag                                                                                                              <  2:448621/150‑1 (MQ=255)
                                               tGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATg                                                                                                    <  2:501695/150‑1 (MQ=255)
                                                     tGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGAACCCACGCCATGTGCTGCTGCGCACCATTGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTAGACCAGCAGCGTGATGCCCAgg                                                                                              <  2:587799/150‑1 (MQ=255)
                                                          cccAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGgccgc                                                                                         >  2:60182/1‑150 (MQ=255)
                                                           ccAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGgccgcc                                                                                        >  2:413104/1‑150 (MQ=255)
                                                            cAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCCGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGccc                                                                                       <  2:242754/150‑1 (MQ=255)
                                                                  gggTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAAcc                                                                                 <  1:60182/150‑1 (MQ=255)
                                                                    gTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCgg                                                                               >  1:106210/1‑150 (MQ=255)
                                                                       tGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCAc                                                                            >  1:437984/1‑150 (MQ=255)
                                                                       tGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTACGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCAc                                                                            >  2:394836/1‑150 (MQ=255)
                                                                           ggCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGa                                                                        >  1:733081/1‑150 (MQ=255)
                                                                              cAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCGGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACaa                                                                     >  1:520526/1‑150 (MQ=255)
                                                                                   tCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTc                                                                                                                    <  1:197776/98‑1 (MQ=255)
                                                                                   tCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTc                                                                                                                    >  2:197776/1‑98 (MQ=255)
                                                                                   tCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATg                                                                <  2:106210/150‑1 (MQ=255)
                                                                                           ccACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATggc                                                              <  2:605049/144‑1 (MQ=255)
                                                                                           ccACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATggc                                                              >  1:605049/1‑144 (MQ=255)
                                                                                                      gctgctgcGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCAc                                             >  1:189875/1‑150 (MQ=255)
                                                                                                          ctgcGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGCGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAg                                         <  1:719139/150‑1 (MQ=255)
                                                                                                          ctgcGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGCGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAg                                         <  1:719076/150‑1 (MQ=255)
                                                                                                                  cATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATg                                                                <  2:155973/119‑1 (MQ=255)
                                                                                                                  cATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATg                                                                >  1:155973/1‑119 (MQ=255)
                                                                                                                     cGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCt                              <  2:189875/150‑1 (MQ=255)
                                                                                                                                 ggCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCCACCTGCTCGAACAGAAAACCGATGCTCAAAGCCGTCCTCAGCCATTACCTTTCTGCGGTGGCGGCCGCGTCCGCGTCACCCTGTTCTATGGCCTGCGCGAACTGGa                  <  2:90211/150‑1 (MQ=255)
                                                                                                                                   ccGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATg                                  >  1:658752/1‑132 (MQ=255)
                                                                                                                                   ccGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATg                                  <  2:658752/132‑1 (MQ=255)
                                                                                                                                   ccGATGGAAATCACCAGGTGATGGCCGGGGATCTGCAGTGCTACATGCTCGAGCAGCAGAGTGCATCACGGCGTCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCTGCGCGAACTGGAGc                <  1:413104/150‑1 (MQ=255)
                                                                                                                                         gTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCTGCGCGAACTGGAGCAGATCg          >  2:166167/1‑150 (MQ=255)
                                                                                                                                            aTCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCTGCGCGAACTGGAGCAGATCGAAc       >  1:153961/1‑150 (MQ=255)
                                                                                                                                                cccGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCt                              >  1:47077/1‑123 (MQ=255)
                                                                                                                                                cccGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCt                              <  2:47077/123‑1 (MQ=255)
                                                                                                                                                 ccGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCTGCGCGAACTGGAGCAGATCGAACCGTTc  <  2:153961/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCTGGTGCGCCTGCCCTGGCGCCGGGTGCCCACCGCCGCAGTGCAGGTGAGCATGCACCCCAGCGAGGGTCTGCCGGCCAGCATCGCGGACCCACGCCATGTGCTGCTGCGCACCATCGAAGCGTTGAAGGCCGATGGTTATCACCCGGTGATGGCCTGCGAGCTGGAGTTCTACCTGCTCGACCAGCAGCGTGATGCCCAGGGCCGCCCACAACCGGCACTGGACAACGATGGCGGCCGCCCCCGCAGCACCCAGGTCTATGGCCTGCGCGAACTGGAGCAGATCGAACCGTTC  >  NC_002947/5168697‑5168991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑