| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,182,887 | A→C | 13.9% | D110A (GAC→GCC) | PP_4562 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,182,887 | 0 | A | C | 13.9% | 80.1 / 9.2 | 36 | D110A (GAC→GCC) | PP_4562 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/17); new base C (3/2); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.84e-01 | |||||||||||
CACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGAC > NC_002947/5182738‑5183034 | cacGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGa > 1:395868/1‑150 (MQ=255) gCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGaca > 2:502129/1‑149 (MQ=255) gCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGaca < 1:502129/149‑1 (MQ=255) aaGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCTGGACTCGCTGCTGCGCGACACCCGTcc > 1:454135/1‑150 (MQ=255) gAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGc < 2:511738/150‑1 (MQ=255) gcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCTCGCCACCCGGACGGGTGTCGcgca > 2:395265/1‑147 (MQ=255) gcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCTCGCCACCCGGACGGGTGTCGcgca < 1:395265/147‑1 (MQ=255) ccTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGa < 1:666650/150‑1 (MQ=255) gAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACtt > 1:672185/1‑150 (MQ=255) aGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTcc < 2:395868/150‑1 (MQ=255) tGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCa > 1:67338/1‑150 (MQ=255) caATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGt > 2:643280/1‑150 (MQ=255) aaGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCTGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTaa < 2:454135/150‑1 (MQ=255) ggCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAgg > 1:34313/1‑150 (MQ=255) tCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCg > 1:1006/1‑150 (MQ=255) tGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGc < 2:421916/150‑1 (MQ=255) gCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCttt < 2:412839/150‑1 (MQ=255) cAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtc < 1:643280/150‑1 (MQ=255) cAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtc < 1:277679/150‑1 (MQ=255) aTGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTCGGCTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCg > 1:86100/1‑150 (MQ=255) gAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCGGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCTCAACTTCGTACGTGCTGAGTTCCGGCAGGCTTTCTCGATg > 2:41015/1‑150 (MQ=255) cgcAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTGTCTCGATGCGATCg > 1:408632/1‑150 (MQ=255) aaGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCa < 1:740005/150‑1 (MQ=255) cTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCAGCTGCTCGCCACCCGGACGGGTg > 2:720631/1‑78 (MQ=25) cTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCAGCTGCTCGCCACCCGGACGGGTg < 1:720631/78‑1 (MQ=25) cgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAgg < 2:438979/150‑1 (MQ=255) cgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAgg < 1:603507/150‑1 (MQ=255) tCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGa > 1:179867/1‑150 (MQ=255) ccTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGcc > 2:436638/1‑150 (MQ=255) cTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAAGCTTTCGCGGTGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCa > 2:419747/1‑150 (MQ=255) cgcCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGc < 2:179867/150‑1 (MQ=255) gggcTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGCTGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGaa < 2:320659/147‑1 (MQ=255) cTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGtt < 2:34313/150‑1 (MQ=255) tccagctgctCGCCACCCGGACGGGTGTCGCGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc > 2:527274/5‑150 (MQ=38) cGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTGCCGCAACTTCGTACATTCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc < 1:408158/150‑1 (MQ=255) ctgctgCGCGACACCCGTCCGGGTGGCGAGCAGATCGAGCGCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGccc < 1:41015/150‑1 (MQ=255) tgctgcGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCt > 2:165760/1‑150 (MQ=255) cgcgACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTg > 2:173765/1‑150 (MQ=255) cgACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGAc < 1:436638/150‑1 (MQ=255) | CACGCCGGAAAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGAC > NC_002947/5182738‑5183034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |