| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 243,623 | T→A | 11.8% | K183I (AAA→ATA) ‡ | fkl ← | FKBP‑type peptidyl‑prolyl cis‑trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 243,623 | 0 | T | A | 11.8% | 98.4 / 5.7 | 34 | K183I (AAA→ATA) ‡ | fkl | FKBP‑type peptidyl‑prolyl cis‑trans isomerase |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base A (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
AGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCGCACTTGCA > NC_002947/243478‑243755 | agagGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGgc < 2:277334/150‑1 (MQ=255) agGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGgcgc < 2:731454/150‑1 (MQ=255) gtgcagtgcaGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTAACAGGCGCCATTTGGCGCCgg > 1:241962/1‑150 (MQ=255) tgcagtgcagGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGgt < 1:36440/150‑1 (MQ=255) gagtgcaaGCCTGAGGTTGGCGTCAGTCGCCAGCCGCCAGTGGTTCTATGTCGAAACCCAAGGGGGTGTAGGGTGCAATCATGTCTCCATCCCCCTCGGCGCCATAGGCTTGCGCTGGAGGAATTACCAGGCGCCATTTCGCGCCGgtgt < 2:75859/149‑1 (MQ=255) cagtgcagGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGgtgt < 1:729183/150‑1 (MQ=255) cTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTAGAAGAGTTTCCAGGCGCCATTTGGCGCCGGTGTGCATTTTCgg > 1:775564/1‑150 (MQ=255) gTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcct > 2:68890/1‑124 (MQ=255) gTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcct < 1:68890/125‑2 (MQ=255) aaCCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGgc > 2:26527/1‑111 (MQ=255) aaCCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGgc < 1:26527/111‑1 (MQ=255) agTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGaa > 1:340309/1‑150 (MQ=255) tctcGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGt < 2:340309/150‑1 (MQ=255) cAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcct < 1:41544/88‑2 (MQ=255) cAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcct > 2:41544/1‑87 (MQ=255) ggTGCAATCAGGTCTCCCGCCCCCTCGGCGCCCTAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCAAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtctggttc > 1:50099/1‑150 (MQ=255) aaTCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTggg > 1:422454/1‑134 (MQ=255) aaTCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTggg < 2:422454/134‑1 (MQ=255) agttctcCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGt < 2:767828/131‑1 (MQ=255) aGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtc > 2:354854/1‑135 (MQ=255) aGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtc < 1:354854/135‑1 (MQ=255) aGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGt > 1:767828/1‑134 (MQ=255) ggTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCa > 1:590157/1‑94 (MQ=255) ggTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCa < 2:590157/94‑1 (MQ=255) tcCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGa < 1:115460/150‑1 (MQ=255) cccGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGAAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAg < 2:775564/150‑1 (MQ=255) ccATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTGGGCGCCGGTGTGCATTTTCGGCAAGGCGGCCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTg > 1:646101/1‑150 (MQ=255) aTAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGcc > 2:601026/1‑150 (MQ=255) aGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCca > 2:642379/1‑150 (MQ=255) tGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCgcac > 2:738604/1‑150 (MQ=255) gAAGGAATTACCAGGCGCCATTTGGCGCCAGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCgcact > 1:623350/1‑150 (MQ=255) aaGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtctgg < 2:631531/101‑1 (MQ=255) aaGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtctgg > 1:631531/1‑101 (MQ=255) gAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCgcacttgca > 2:439299/1‑150 (MQ=255) | AGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCGCACTTGCA > NC_002947/243478‑243755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |