Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,182,894	T→G	13.6%	R112R (CGT→CGG)	PP_4562 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,182,894	0	T	G	13.6%	76.9 / 9.0	37	R112R (CGT→CGG)	PP_4562	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/17);  new base G (3/2);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

AAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAA  >  NC_002947/5182747‑5183040                                                                                                                                                    |                                                                                                                                                   aaGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCTGGACTCGCTGCTGCGCGACACCCGTcc                                                                                                                                                  >  1:454135/1‑150 (MQ=255)        gAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGc                                                                                                                                           <  2:511738/150‑1 (MQ=255)                gcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCTCGCCACCCGGACGGGTGTCGcgca                                                                                                                                      >  2:395265/1‑147 (MQ=255)                gcaCCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCTCGCCACCCGGACGGGTGTCGcgca                                                                                                                                      <  1:395265/147‑1 (MQ=255)                   ccTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGa                                                                                                                                <  1:666650/150‑1 (MQ=255)                      gAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACtt                                                                                                                             >  1:672185/1‑150 (MQ=255)                        aGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTcc                                                                                                                           <  2:395868/150‑1 (MQ=255)                           tGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCa                                                                                                                        >  1:67338/1‑150 (MQ=255)                                  caATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGt                                                                                                                 >  2:643280/1‑150 (MQ=255)                                            aaGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCTGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTaa                                                                                                       <  2:454135/150‑1 (MQ=255)                                              ggCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAgg                                                                                                     >  1:34313/1‑150 (MQ=255)                                                 tCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCg                                                                                                  >  1:1006/1‑150 (MQ=255)                                                       tGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGc                                                                                            <  2:421916/150‑1 (MQ=255)                                                          gCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCttt                                                                                         <  2:412839/150‑1 (MQ=255)                                                           cAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtc                                                                                        <  1:277679/150‑1 (MQ=255)                                                           cAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTtc                                                                                        <  1:643280/150‑1 (MQ=255)                                                              aTGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTCGGCTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCg                                                                                     >  1:86100/1‑150 (MQ=255)                                                                 gAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCGGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCTCAACTTCGTACGTGCTGAGTTCCGGCAGGCTTTCTCGATg                                                                                  >  2:41015/1‑150 (MQ=255)                                                                       cgcAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTGTCTCGATGCGATCg                                                                            >  1:408632/1‑150 (MQ=255)                                                                          aaGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCa                                                                         <  1:740005/150‑1 (MQ=255)                                                                              cTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCAGCTGCTCGCCACCCGGACGGGTg                                                                                                                                             <  1:720631/78‑1 (MQ=25)                                                                              cTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCAGCTGCTCGCCACCCGGACGGGTg                                                                                                                                             >  2:720631/1‑78 (MQ=25)                                                                                         cgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAgg                                                          <  2:438979/150‑1 (MQ=255)                                                                                         cgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAgg                                                          <  1:603507/150‑1 (MQ=255)                                                                                                   tCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGa                                                >  1:179867/1‑150 (MQ=255)                                                                                                             ccTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGcc                                      >  2:436638/1‑150 (MQ=255)                                                                                                              cTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAAGCTTTCGCGGTGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCa                                     >  2:419747/1‑150 (MQ=255)                                                                                                                   cgcCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGc                                <  2:179867/150‑1 (MQ=255)                                                                                                                            gggcTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGCTGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGaa                       <  2:320659/147‑1 (MQ=255)                                                                                                                               cTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGtt                    <  2:34313/150‑1 (MQ=255)                                                                                                                                 tccagctgctCGCCACCCGGACGGGTGTCGCGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc                  >  2:527274/5‑150 (MQ=38)                                                                                                                                 cGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTGCCGCAACTTCGTACATTCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc                  <  1:408158/150‑1 (MQ=255)                                                                                                                                   ctgctgCGCGACACCCGTCCGGGTGGCGAGCAGATCGAGCGCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGccc                <  1:41015/150‑1 (MQ=255)                                                                                                                                    tgctgcGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCt               >  2:165760/1‑150 (MQ=255)                                                                                                                                         cgcgACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTg          >  2:173765/1‑150 (MQ=255)                                                                                                                                           cgACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGAc        <  1:436638/150‑1 (MQ=255)                                                                                                                                                 ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTTCCCTTGCTGACGTTaaa  >  1:93190/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AAGCGATGAGCTGCAGCACCTGAAGGTGCCCACCAATTTCCTCAAGGCTCACCTTGAGCAAATGGAAGTGCGCAAGCCTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAA  >  NC_002947/5182747‑5183040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑