Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,324,205 T→G 12.5% L264R (CTG→CGG)  PP_4684 → kinase‑like domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,324,2050TG12.5% 66.9 / 3.4 30L264R (CTG→CGG) PP_4684kinase‑like domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (15/11);  new base G (2/2);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

ATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCGT  >  NC_002947/5324060‑5324353
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aTCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGcc                                                                                                                                                  >  1:792648/1‑150 (MQ=255)
      cTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgcgc                                                                                                                                            >  1:469737/1‑150 (MQ=255)
          gCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGACGTGCCTGGCCCGGCGCGtca                                                                                                                                        >  2:50691/1‑150 (MQ=255)
           cAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtcag                                                                                                                                       <  2:51325/150‑2 (MQ=255)
             aCGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCCCGGCCTGTAGTGCCTGGGCCGGCGCTtcatca                                                                                                                                     <  1:50691/150‑1 (MQ=255)
                    cTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTa                                                                                                                              >  1:22472/1‑150 (MQ=255)
                        aTCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTg                                                                                                                          <  2:22472/150‑1 (MQ=255)
                         tCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTgg                                                                                                                         >  2:310190/1‑150 (MQ=255)
                            aTGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAAc                                                                                                                      >  1:664693/1‑150 (MQ=255)
                            aTGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAAc                                                                                                                      <  1:310190/150‑1 (MQ=255)
                                                   tGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTg                                                                                               <  2:792648/150‑1 (MQ=255)
                                                          aGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGc                                                                                        <  1:451766/150‑1 (MQ=255)
                                                              cAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCaa                                                                                    <  1:195001/150‑1 (MQ=255)
                                                               aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCa                                                                                                                                 >  1:697407/1‑104 (MQ=255)
                                                               aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCa                                                                                                                                 <  2:697407/104‑1 (MQ=255)
                                                               aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAAc                                                                                   >  2:353533/1‑150 (MQ=255)
                                                                 cGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACtt                                                                                 <  2:765950/150‑1 (MQ=255)
                                                                                gACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACcg                                                                  >  2:53167/1‑150 (MQ=255)
                                                                                          tACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTa                                                        >  1:263600/1‑150 (MQ=255)
                                                                                                        cTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                        <  2:299491/56‑1 (MQ=21)
                                                                                                        cTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                        >  1:299491/1‑56 (MQ=21)
                                                                                                               gCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGaa                                                                                                     <  1:77810/84‑1 (MQ=255)
                                                                                                               gCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGaa                                                                                                     >  2:77810/1‑84 (MQ=255)
                                                                                                                      aCCTGGAAGACCGCGGCCTGAAATGCCGGGCCCGGCGCTTCAAGAGCCAGTACCTAGAACTGACTGGGGAGTACGAAGGCCTGGAACTGCTCAACATCTACGAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCCGCATgc                            >  2:533062/1‑150 (MQ=255)
                                                                                                                                    ggCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGCTCAGCATGCCGTCCGACGCTGAt              <  2:469737/150‑1 (MQ=255)
                                                                                                                                       cTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTg                <  2:366890/145‑1 (MQ=255)
                                                                                                                                       cTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTg                >  1:366890/1‑145 (MQ=255)
                                                                                                                                        tGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCg          >  1:260653/1‑150 (MQ=255)
                                                                                                                                               ccTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCg   >  1:370766/1‑150 (MQ=255)
                                                                                                                                                cTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGCTTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCGt  >  1:361976/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCGT  >  NC_002947/5324060‑5324353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑