Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,338,098 C→G 14.8% intergenic (+57/‑227) PP_4696 → / → PP_mr53 nitrogen regulation protein NR(I)/CrcZ ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,338,0980CG14.8% 67.4 / 5.7 27intergenic (+57/‑227)PP_4696/PP_mr53nitrogen regulation protein NR(I)/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base C (12/11);  new base G (2/2);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

ACGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTG  >  NC_002947/5337951‑5338246
                                                                                                                                                   |                                                                                                                                                    
aCGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCTCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGccgcccg                                                                                                                                                    <  2:271818/150‑1 (MQ=255)
       cTCGGCGTAAGCCGCAAGTGTCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCCCCCCCACACCGCACGCCGCAGCTTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCgcggcg                                                                                                                                             <  2:518181/150‑1 (MQ=255)
        tCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGGCGgcgc                                                                                                                                            >  1:397842/1‑150 (MQ=255)
          ggCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                          >  1:315463/1‑150 (MQ=255)
              tAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGGCGGCGCTCggtc                                                                                                                                      <  2:397842/150‑3 (MQ=255)
                               tgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgt                                                                                                                     <  1:113280/150‑1 (MQ=255)
                                                  tGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCAGTGAGACCGAGCACCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTGACGACGAACACCCCAg                                                                                                  >  1:272898/1‑150 (MQ=255)
                                                          ccACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacc                                                                                          >  1:428959/1‑150 (MQ=255)
                                                                  cAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacctc                                                                                        >  1:243900/1‑144 (MQ=255)
                                                                  cAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacctc                                                                                        <  2:243900/144‑1 (MQ=255)
                                                                  cAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTcc                                                                                  <  2:660648/150‑1 (MQ=255)
                                                                     gagCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                         >  2:537068/1‑92 (MQ=255)
                                                                     gagCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                         <  1:537068/92‑1 (MQ=255)
                                                                         aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaaa                                                                           >  1:576161/1‑150 (MQ=255)
                                                                               aCCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGt                                                                     <  2:576161/150‑1 (MQ=255)
                                                                                 cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgac                                                                                                             >  1:64881/1‑108 (MQ=255)
                                                                                 cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgac                                                                                                             <  2:64881/108‑1 (MQ=255)
                                                                                 cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTa                                                                   >  2:68141/1‑150 (MQ=255)
                                                                                      aaTAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCaa                                                              <  2:428959/150‑1 (MQ=255)
                                                                                            ccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGaa                                                                                                          >  2:504034/1‑100 (MQ=255)
                                                                                            ccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGaa                                                                                                          <  1:504034/100‑1 (MQ=255)
                                                                                              aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccg                                                      >  2:321133/1‑150 (MQ=255)
                                                                                                        cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTaa                                                                                              <  2:645593/100‑1 (MQ=255)
                                                                                                        cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGAGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTaa                                                                                              >  1:645593/1‑100 (MQ=255)
                                                                                                         gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTc                                           >  1:4778/1‑150 (MQ=255)
                                                                                                               cgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGa                                      >  2:249367/1‑149 (MQ=255)
                                                                                                               cgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGa                                      <  1:249367/149‑1 (MQ=255)
                                                                                                                              gTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGaa                      <  2:4778/150‑1 (MQ=255)
                                                                                                                                       ctgtgaggccgccCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACa             >  2:27646/8‑150 (MQ=255)
                                                                                                                                                  cccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTg  <  1:68141/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTG  >  NC_002947/5337951‑5338246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑