Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,364,611 A→T 16.7% V24D (GTC→GAC)  folP ← dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,364,6110AT16.7% 68.2 / 8.2 30V24D (GTC→GAC) folPdihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base A (13/12);  new base T (3/2);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

CCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAGGC  >  NC_002947/5364467‑5364750
                                                                                                                                                |                                                                                                                                           
cccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGTCATgg                                                                                                                                        <  1:464054/150‑1 (MQ=255)
 ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATggg                                                                                                                                       >  1:9166/1‑150 (MQ=255)
 ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATggg                                                                                                                                       >  2:540760/1‑150 (MQ=255)
     gcgcGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCACCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATg                                                                                                                                   <  2:715338/150‑1 (MQ=255)
                  ccACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGa                                                                                                                      <  1:352036/150‑1 (MQ=255)
                    aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATg                                                                                                                                   >  1:363110/1‑135 (MQ=255)
                    aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATg                                                                                                                                   <  2:363110/135‑1 (MQ=255)
                                         cccGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGCCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGCGTGATATTGAGAATACCCATTACCTGGGTATCCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCg                                                                                               <  2:242821/149‑1 (MQ=255)
                                               ggcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg                                                                                         <  2:151912/150‑1 (MQ=255)
                                                     cATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggt                                                                                            >  2:494537/1‑141 (MQ=255)
                                                     cATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggt                                                                                            <  1:494537/141‑1 (MQ=255)
                                                                ctgtgcgcATTGCCGCGGCGCGGTGAATGGCGCGCACGCCCACGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCa                                                                        <  2:729695/146‑1 (MQ=255)
                                                                cGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCa                                                                        <  1:540760/150‑1 (MQ=255)
                                                                      gcAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATg                                                                  >  2:615900/1‑150 (MQ=255)
                                                                      gcAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGACAGGAAACCGGGTAGGGTACGGCACTGAGCTCATAGATg                                                                  >  1:449864/1‑150 (MQ=255)
                                                                                              aGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGgcc                                          <  2:682575/150‑1 (MQ=255)
                                                                                                        aTCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGc                                <  2:9166/150‑1 (MQ=255)
                                                                                                            gagaAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg                                                                                         >  2:355852/1‑89 (MQ=255)
                                                                                                            gagaAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg                                                                                         <  1:355852/89‑1 (MQ=255)
                                                                                                             agaAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTc                           >  2:404639/1‑150 (MQ=255)
                                                                                                               aaGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCg                         >  2:504732/1‑150 (MQ=255)
                                                                                                                 ggAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGa                       >  1:633949/1‑150 (MQ=255)
                                                                                                                     tCGGGGGTGATATTGAGAATACCCATGACATGGGTATGTGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACt                                                                               >  2:11269/1‑90 (MQ=255)
                                                                                                                     tCGGGGGTGATATTGAGAATACCCATGACATGGGTATGTGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACt                                                                               <  1:11269/90‑1 (MQ=255)
                                                                                                                     tCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCt                   >  1:783497/1‑150 (MQ=255)
                                                                                                                        ggggTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCAt                >  1:630595/1‑150 (MQ=255)
                                                                                                                                     gcaTACCCATGTCATGGGTATTCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAgg   >  1:670885/3‑150 (MQ=255)
                                                                                                                                      caTACCCATGTCATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTTCTTTCGTAGATGCCCTTACAACTCAGCGGATGAGCCGCCGTTTGACGATTCCGGACGCTCCTCCTGCGCCCCAAGc  >  1:776117/2‑150 (MQ=255)
                                                                                                                                          cccATGTCATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGa                                                                             >  2:398248/1‑71 (MQ=255)
                                                                                                                                          cccATGTCATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGa                                                                             <  1:398248/71‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
CCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAGGC  >  NC_002947/5364467‑5364750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑