Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,420,010 G→C 8.5% R216R (CGG→CGC)  PP_4759 → transcriptional regulator, GntR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,420,0100GC 8.5% 148.0 / 4.8 47R216R (CGG→CGC) PP_4759transcriptional regulator, GntR family
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base C (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTGCAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCA  >  NC_002947/5419861‑5420133
                                                                                                                                                     |                                                                                                                           
ctgcAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgg                                                                                                                             >  1:284745/1‑150 (MQ=255)
    gaCTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgggcgg                                                                                                                         >  2:547214/2‑150 (MQ=255)
       tGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTAACTGGTCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGCGGCat                                                                                                                      >  1:215952/1‑149 (MQ=255)
          gTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAgtgt                                                                                                                   <  2:475501/150‑1 (MQ=255)
           tAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGCGGCatccgg                                                                                                                  <  2:215952/150‑6 (MQ=255)
             aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgg                                                                                                                             >  1:615242/1‑137 (MQ=255)
             aCGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCgcgg                                                                                                                             <  2:615242/137‑1 (MQ=255)
                 ccTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCa                                                                                                            <  2:95224/150‑1 (MQ=255)
                  cTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCAc                                                                                                           >  2:638384/1‑150 (MQ=255)
                  cTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGCGGCatccgg                                                                                                                  >  1:44521/1‑138 (MQ=255)
                  cTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCCCGCGCGGCatccgg                                                                                                                  <  2:44521/143‑6 (MQ=255)
                        ccGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACATGTcc                                                                                                     >  1:557247/1‑150 (MQ=255)
                              aaCCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGc                                                                                               >  1:146478/1‑150 (MQ=255)
                                 cGCATGACCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCga                                                                                            >  2:282660/1‑150 (MQ=255)
                                    aTGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGAc                                                                                         <  1:547214/150‑1 (MQ=255)
                                    aTGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGAc                                                                                         <  2:146478/150‑1 (MQ=255)
                                         ccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAgtg                                                                                                                    >  2:627429/1‑118 (MQ=255)
                                         ccAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAgtg                                                                                                                    <  1:627429/118‑1 (MQ=255)
                                                      gcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACa                                                                                                 <  1:28029/124‑1 (MQ=255)
                                                      gcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACa                                                                                                 >  2:28029/1‑124 (MQ=255)
                                                      gcgGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGgactga                                                                       <  1:415085/150‑1 (MQ=255)
                                                              cAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAgg                                                               <  2:163788/150‑1 (MQ=255)
                                                               aaGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTa                                                                                   <  1:259212/129‑1 (MQ=255)
                                                               aaGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTa                                                                                   >  2:259212/1‑129 (MQ=255)
                                                               aaGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGc                                                              >  1:784549/1‑150 (MQ=255)
                                                                    ggCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgtgt                                                         <  2:284745/150‑1 (MQ=255)
                                                                                   ccTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTg                                          <  1:525581/150‑1 (MQ=255)
                                                                                     tGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGc                                        <  1:638384/150‑1 (MQ=255)
                                                                                          aaCCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                   <  2:784549/150‑1 (MQ=255)
                                                                                             cTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAggg                                >  2:784603/1‑150 (MQ=255)
                                                                                               ggAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTg                              >  1:552131/1‑150 (MQ=255)
                                                                                                     tGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGgact                                                                         >  1:498460/1‑101 (MQ=255)
                                                                                                     tGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGgact                                                                         <  2:498460/101‑1 (MQ=255)
                                                                                                           gATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATcc                  >  1:74956/1‑150 (MQ=255)
                                                                                                           gATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATcc                  >  2:528095/1‑150 (MQ=255)
                                                                                                           gATCCTCAATGCCATCCGCCAACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATcc                  <  1:150901/150‑1 (MQ=255)
                                                                                                            aTCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                   >  2:70071/1‑132 (MQ=255)
                                                                                                            aTCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCa                                   <  1:70071/132‑1 (MQ=255)
                                                                                                                tCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgt                                                           >  2:432566/1‑104 (MQ=255)
                                                                                                                tCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgt                                                           <  1:432566/104‑1 (MQ=255)
                                                                                                                     gCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACATGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTg        <  2:557247/150‑1 (MQ=255)
                                                                                                                       cATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgt                                                           <  2:256981/97‑1 (MQ=255)
                                                                                                                       cATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAgt                                                           >  1:256981/1‑97 (MQ=255)
                                                                                                                       cATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGcc      >  1:567309/1‑150 (MQ=255)
                                                                                                                        aTCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTg                                          >  1:129380/1‑113 (MQ=255)
                                                                                                                        aTCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTg                                          <  2:129380/113‑1 (MQ=255)
                                                                                                                        aTCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGccc     >  1:667479/1‑150 (MQ=255)
                                                                                                                        aTCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGccc     >  2:123315/1‑150 (MQ=255)
                                                                                                                           cgccgACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCa  <  1:123315/150‑1 (MQ=255)
                                                                                                                                    gACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGcc                                                                                              <  1:444202/49‑1 (MQ=255)
                                                                                                                                    gACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGcc                                                                                              >  2:444202/1‑49 (MQ=255)
                                                                                                                                                     |                                                                                                                           
CTGCAACTGGGTAACGGCCTGATCCCGCGCAACCGCATGGCCCAGGCCGAGCGCGCGGGGGCCAAGCTGGCGCGGCAAGCGTACCTGGCCAACCTGGAGCATGAGGCGATCCTCAATGCCATCCGCCGACAAGACCCGGATGCCGCGCGGGCGGCAGTGTGCCTGCACCTGTCCAACAGCCGAGACCGGCTACTGCCGGACTGACTGTCAGGCAGTGTAAGACGAGTATATTGGCGTGCAGGGTGGCCGTTACATCCAGCTACCCTGCCCTCA  >  NC_002947/5419861‑5420133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑