| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,471,540 | T→C | 9.1% | E138G (GAG→GGG) | mrdA‑II ← | transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,471,540 | 0 | T | C | 9.1% | 119.5 / 3.4 | 44 | E138G (GAG→GGG) | mrdA‑II | transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (20/20); new base C (2/2); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
GTTGATCCGCCCGACATAACCCACCGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGTTACCCGCACGTTCGCG > NC_002947/5471401‑5471679 | gTTGATCCGCCCGACATAACCCACCGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGGCGCAGCCTCTACACTCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGAt > 1:695296/1‑150 (MQ=255) ccGCCCGACATAACCCACCGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCAc < 1:344360/150‑1 (MQ=255) cccGACATAACCCACCGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCgg < 1:720521/150‑1 (MQ=255) cccGACATAACCCACCGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCgg < 1:720600/150‑1 (MQ=255) cACCGAATGGGCGAAATGCGCACCCTGAAGGTAATGCCCCACCAGCTGTGCCGCCACTTAACAACCCGGTAAGCGCAGCCGAGATCTTCG‑GAGATGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCCCCGGCCCTAATGGCcg > 1:380037/1‑150 (MQ=255) cGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCcggcg > 1:540971/1‑150 (MQ=255) cGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCcggcg < 2:540971/150‑1 (MQ=255) gAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCcggcgg > 1:750257/1‑150 (MQ=255) tGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATg > 1:420886/1‑150 (MQ=255) tGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATg < 2:420886/150‑1 (MQ=255) cgcACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACATCCACACCAGGCAGGTGCTGCTG‑GGTGACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATgcg < 1:575929/150‑1 (MQ=255) gcACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCAAATTGCCGGCGGCCCTGGCGCATgcgc > 1:241312/1‑150 (MQ=255) ccTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCCCGAACAGGATCGGCACCGGc < 2:452484/116‑1 (MQ=255) ccTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCCCGAACAGGATCGGCACCGGc > 1:452476/1‑116 (MQ=255) ccTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCCCGAACAGGATCGGCACCGGc > 1:452484/1‑116 (MQ=255) ccTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCCCGAACAGGATCGGCACCGGc < 2:452476/116‑1 (MQ=255) tGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCg < 1:24522/150‑1 (MQ=255) gTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAg < 1:499823/150‑1 (MQ=255) gCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCAc > 1:740917/1‑150 (MQ=255) ccGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACg > 1:205381/1‑150 (MQ=255) tgCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGt < 1:418295/150‑1 (MQ=255) caccacCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAg > 1:200304/1‑150 (MQ=255) ccaccTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGt > 1:656479/1‑150 (MQ=255) caccTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTc > 1:612288/1‑150 (MQ=255) accTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAAc < 2:86213/69‑1 (MQ=255) accTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAAc > 1:86213/1‑69 (MQ=255) accTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCAc < 1:535097/80‑1 (MQ=255) accTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCAc > 2:535097/1‑80 (MQ=255) acCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTc > 2:308462/1‑150 (MQ=255) cGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGAACCGGCTCGAATGGCCGGCGGCCCTGgc < 2:311225/95‑1 (MQ=255) cGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGAACCGGCTCGAATGGCCGGCGGCCCTGgc > 1:311225/1‑95 (MQ=255) aGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCAc < 1:858/140‑1 (MQ=255) aGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCAc > 2:858/1‑140 (MQ=255) aGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATg < 2:656479/150‑1 (MQ=255) aCTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCa < 2:200304/150‑1 (MQ=255) aCTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCa < 2:380037/150‑1 (MQ=255) tG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGc > 2:780717/1‑150 (MQ=255) gcgGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGtt < 1:136811/150‑1 (MQ=255) tCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGTTACCCGCAc < 1:780717/150‑1 (MQ=255) gcgctTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGTTACCCGCACGtt > 1:797176/4‑150 (MQ=255) ctTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGTTACCCGCACGTTcgc > 1:709292/1‑150 (MQ=255) ttCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGTTACCCGCACGTTcgcg < 2:205381/150‑1 (MQ=255) cTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGc > 2:114399/1‑121 (MQ=255) cTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGc < 1:114399/121‑1 (MQ=255) | GTTGATCCGCCCGACATAACCCACCGAATGGGCGAAATGCGCACCCTGCGGGTAATGCCGCACCAGCTGTGCCACCACCTCGACACCCGGCAGGCGGAACTG‑GTTTACCGCCACGCGGGCGATCTGCTCTTCGTTGAGCTCGAACAGGATCGGCACCGGCTCGAATGGCCGGCGGCCCTGGCGCATGCGCTTCTCGAACAGGGCACGGTCGTCAGCGGTGAGGTCCAGCACTTCGACGATGGTATCCAGCACTTCCTGCCAGTTACCCGCACGTTCGCG > NC_002947/5471401‑5471679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |