| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,525,818 | C→A | 12.9% | intergenic (+60/‑63) | PP_4858 → / → PP_4859 | hypothetical protein/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,525,818 | 0 | C | A | 12.9% | 87.3 / 3.6 | 31 | intergenic (+60/‑63) | PP_4858/PP_4859 | hypothetical protein/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (13/14); new base A (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
TGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGTTTC > NC_002947/5525688‑5525967 | tGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGtttt > 1:66762/1‑150 (MQ=255) gAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTAt < 1:321706/150‑1 (MQ=255) gAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTAt < 2:667351/150‑1 (MQ=255) gCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCt < 2:579458/146‑1 (MQ=255) gCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCt > 1:579458/1‑146 (MQ=255) tCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCAtt < 1:338624/113‑1 (MQ=255) tCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCAtt > 2:338624/1‑113 (MQ=255) gCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCAATGGAc < 2:676740/114‑1 (MQ=255) gCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGcat < 2:66762/150‑1 (MQ=255) ccTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGcatc > 1:711454/1‑150 (MQ=255) aaCTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGcc > 1:109481/1‑150 (MQ=255) cAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCcgcgc < 2:216254/150‑1 (MQ=255) cGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGtttttt < 2:611914/71‑1 (MQ=255) cGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGtttttt > 1:611914/1‑71 (MQ=255) cGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTCACCGTTTCAGACTCCCGTATTCTGCTGCCGCGTGCCGACCCCAACCTATAGGCCCGCCa > 2:352155/1‑150 (MQ=255) aaaGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCa > 2:770817/1‑147 (MQ=255) aaaGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCa < 1:770817/147‑1 (MQ=255) aacaacTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTg > 2:3623/1‑150 (MQ=255) ttCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGAt > 1:408451/1‑150 (MQ=255) aaCGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGaa > 1:510601/1‑108 (MQ=255) aaCGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGaa < 2:510601/108‑1 (MQ=255) cccTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGga < 2:711454/150‑1 (MQ=255) cTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTa < 1:334864/63‑1 (MQ=255) cTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTa > 2:334864/1‑63 (MQ=255) cAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCGGACCGTTACAGAAGCCCGCATCATGCTGCCTCGCGCCGAACATAAGCTACATACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTg < 2:164550/150‑1 (MQ=255) aGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTgg < 1:655809/150‑1 (MQ=255) agCCCGGTCAATGGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGc < 2:340645/64‑1 (MQ=38) agCCCGGTCAATGGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGc > 1:340645/2‑65 (MQ=38) agCCCGGTCAATGGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGc > 1:427262/2‑150 (MQ=255) gCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTAc > 2:421606/1‑99 (MQ=255) gCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTAc < 1:421606/99‑1 (MQ=255) ccATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGttt < 2:109481/150‑1 (MQ=255) cATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGTTTc > 1:711091/1‑150 (MQ=255) | TGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGTTTC > NC_002947/5525688‑5525967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |