Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,581,752 T→G 9.7% F246V (TTC→GTC)  PP_4910 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,581,7520TG 9.7% 107.2 / 4.1 41F246V (TTC→GTC) PP_4910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/17);  new base G (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TGCGCGACCCGCAGCCCTATACCCCGGCCACCGACCGCCAGGACGAGATTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCAAAGCCGCCGCAGAGGTCGAGTCCGACGAG  >  NC_002947/5581618‑5581901
                                                                                                                                      |                                                                                                                                                     
tGCGCGACCCGCAGCCCTATACCCCGGCCACCGACCGCCAGGACGAGATTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACg                                                                                                                                        >  1:319381/1‑150 (MQ=255)
             gCCCTATACCCCGGCCACCGACCGCCAGGACGAGATTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGc                                                                                                                           <  2:760911/150‑1 (MQ=255)
              cccTATACCCCGGCCACCGACCGCCAGGACGAGATTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCa                                                                                                                          >  1:206056/1‑150 (MQ=255)
                  ataCCCCGGCCACCGACCGCCAGGACGAGATTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCAacct                                                                                                                      <  2:206056/150‑2 (MQ=255)
                                          atgagaTTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTg                                                                                              <  1:356434/148‑1 (MQ=255)
                                              gaTTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCACCCCCGGAGCCGTAACCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCt                                                                                          >  2:482352/1‑150 (MQ=255)
                                                        aTCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAg                                                                                >  1:24055/1‑150 (MQ=255)
                                                                 cAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATgacgac                                                                       <  2:746639/150‑1 (MQ=255)
                                                                        atgcaCGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGCAGACGACGACGCGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGCTGCCCTGGAAGACGAGGATGACGACGAAGCCt                                                                >  2:731337/1‑150 (MQ=255)
                                                                          gcaCGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAgg                                                                               <  1:99798/133‑1 (MQ=255)
                                                                          gcaCGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAgg                                                                               >  2:99798/1‑133 (MQ=255)
                                                                          gcaCGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTc                                                              >  1:767166/1‑150 (MQ=255)
                                                                               ccTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCTGCGGCCCCGGCACCCAAGGcc                                                                                                                 <  2:780819/94‑1 (MQ=255)
                                                                               ccTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCTGCGGCCCCGGCACCCAAGGcc                                                                                                                 >  1:780819/1‑94 (MQ=255)
                                                                                   gCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGaa                                                                                      >  2:579888/1‑117 (MQ=255)
                                                                                   gCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGaa                                                                                      <  1:579888/117‑1 (MQ=255)
                                                                                     cccGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGa                                                                              <  2:117680/123‑1 (MQ=255)
                                                                                     cccGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGa                                                                              >  1:117680/1‑123 (MQ=255)
                                                                                     cccGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCctgct                                                   <  2:767166/150‑1 (MQ=255)
                                                                                       cGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGc                                                        >  1:36438/1‑143 (MQ=255)
                                                                                       cGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGc                                                        <  2:36438/143‑1 (MQ=255)
                                                                                        gCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGga                                                >  1:176243/1‑150 (MQ=255)
                                                                                          cTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATgacga                                                                        <  1:684655/124‑1 (MQ=255)
                                                                                          cTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATgacga                                                                        >  2:684655/1‑124 (MQ=255)
                                                                                             cGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGgacgacg                                           >  2:503289/1‑150 (MQ=255)
                                                                                              gCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGgacgacga                                          >  2:453375/1‑150 (MQ=255)
                                                                                                  ccggagccggagCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAg                                                                                >  1:110707/1‑108 (MQ=255)
                                                                                                  ccggagccggagCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAg                                                                                <  2:110707/108‑1 (MQ=255)
                                                                                                    ggagccggagccCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACCGc                                    <  1:797164/150‑1 (MQ=255)
                                                                                                      agccggagccCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGccc                                  <  2:319381/150‑1 (MQ=255)
                                                                                                           gggccCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCaaa                             <  1:35214/148‑1 (MQ=255)
                                                                                                           gagccCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCaaa                             >  2:521606/1‑150 (MQ=255)
                                                                                                           gagccCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCaaa                             >  1:496290/1‑150 (MQ=255)
                                                                                                                       gacgacgacGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCAAAGCCGCCGCagag                 <  2:579564/150‑1 (MQ=255)
                                                                                                                          gacgacgaGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATga                                                                           <  2:250138/89‑1 (MQ=255)
                                                                                                                          gacgacgaGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATga                                                                           >  1:250138/1‑89 (MQ=255)
                                                                                                                          gacgacgaGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCAAAGCCGCCGCAGAGGTc              >  2:524513/1‑150 (MQ=255)
                                                                                                                                gtgCAGGTCGTCGAACTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCctgct                                                   >  2:126886/3‑107 (MQ=255)
                                                                                                                                gtgCAGGTCGTCGAACTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCctgct                                                   <  1:126886/105‑1 (MQ=255)
                                                                                                                                 tgCAGGTCGTCGAACTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCAAAGCCGCCGCAGAGGTCGAGTc         >  1:469782/2‑148 (MQ=255)
                                                                                                                                 tgCAGGTCGTCGAACTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCACCAAAGCCGCCGCAGAGGTCGAGTc         <  2:469782/147‑1 (MQ=255)
                                                                                                                                      ttCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCAAAGCCGCCGCAGAGGTCGAGTCCGACGAg  >  2:206259/1‑150 (MQ=255)
                                                                                                                                      |                                                                                                                                                     
TGCGCGACCCGCAGCCCTATACCCCGGCCACCGACCGCCAGGACGAGATTGGCGACATCGCCCGCCAGCTGCATGCACGCCTGGCCCCGCCTCCGCCCCCGGAGCCGGAGCCCGAGGAAGACGACGACGAGCAGTTCGACGACCTGCACGCAGCGGCCCCGGCACCCAAGGCCGCACCGCGCGCCAAGGTTGCCCTGGAAGACGAGGATGACGACGAAGCCTTCGCCGGCCTGCTGGACGACGACACTGCCCCCAAAGCCGCCGCAGAGGTCGAGTCCGACGAG  >  NC_002947/5581618‑5581901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑