Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,624,802 C→G 9.8% G337A (GGG→GCG)  PP_4943 ← glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,624,8020CG 9.8% 125.2 / 5.3 41G337A (GGG→GCG) PP_4943glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (21/16);  new base G (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GGAAGGCCTGGATACGATTGAGCATCAGGCCTGCCCCACCAACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTC  >  NC_002947/5624657‑5624947
                                                                                                                                                  |                                                                                                                                                 
ggAAGGCCTGGATACGATTGAGCATCAGGCCTGCCCCACCAACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGc                                                                                                                                               <  1:545740/150‑1 (MQ=255)
     gCCTGGATACGATTGAGCATCAGGCCTGCCCCACCAACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCa                                                                                                                                          <  2:755427/150‑1 (MQ=255)
           aTACGATTGAGCATCAGGCCTGCCCCACCAACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACgg                                                                                                                                    <  1:146988/150‑1 (MQ=255)
                                   ccaccaACGCGCGCACCTGTGGGAGTTNCCAGAAGGCCTCNCGNACCNCCTGATCAGAGAAACGCTCACCCAGGTGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCa                                                                                                            >  1:696035/1‑150 (MQ=255)
                                    caccaACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCGGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAg                                                                                                           >  2:15748/1‑150 (MQ=255)
                                       caACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGtgct                                                                                                                           >  1:236881/1‑131 (MQ=255)
                                       caACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGtgct                                                                                                                           <  2:236881/131‑1 (MQ=255)
                                       caACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTg                                                                                                        >  1:87925/1‑150 (MQ=255)
                                       caACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTg                                                                                                        >  2:43674/1‑150 (MQ=255)
                                       caACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTg                                                                                                        >  2:199952/1‑150 (MQ=255)
                                              cgcACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGtgc                                                                                                                            <  1:191037/123‑1 (MQ=255)
                                              cgcACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGtgc                                                                                                                            >  2:191037/1‑123 (MQ=255)
                                                 aCCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTTACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCGCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCg                                                                                              >  1:449963/1‑150 (MQ=255)
                                                 aCCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGACGCTGGGCAGCgcca                                                                                                                                           <  2:319762/106‑4 (MQ=255)
                                                 aCCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGGACGCTGGGCAGCgcca                                                                                                                                           >  1:319762/1‑103 (MQ=255)
                                                                   aaGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACg                                                                            >  2:515655/1‑150 (MQ=255)
                                                                       ccTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTg                                                                                                                 >  1:496153/1‑109 (MQ=255)
                                                                       ccTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTg                                                                                                                 <  2:496153/109‑1 (MQ=255)
                                                                        cTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGNCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAAc                                                                       <  2:696035/150‑1 (MQ=255)
                                                                              cACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTc                                                                 <  1:199952/150‑1 (MQ=255)
                                                                                ccGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACCGCCATGTGCTGCAGGCCCTGGGCCAGCCGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGa                                                               <  2:191630/150‑1 (MQ=255)
                                                                                 cGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCa                                                                              >  1:6986/1‑134 (MQ=255)
                                                                                 cGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCa                                                                              <  2:6986/134‑1 (MQ=255)
                                                                                  gCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGAcc                                                             >  1:666433/1‑150 (MQ=255)
                                                                                         cAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTcgc                                                      <  1:515655/150‑1 (MQ=255)
                                                                                          agagAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCCGCGCGCCCCGCACGCCAACCCCCACGACCACTCcgcg                                                     >  2:100185/1‑150 (MQ=255)
                                                                                          agagAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTcgcg                                                     >  2:624291/1‑150 (MQ=255)
                                                                                                  gCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCa                                                                                                                                          <  1:534077/57‑1 (MQ=255)
                                                                                                  gCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCa                                                                                                                                          >  2:534077/1‑57 (MQ=255)
                                                                                                  gCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGc                                             >  2:464339/1‑150 (MQ=255)
                                                                                                            gcgcTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGt                                   <  1:43674/150‑1 (MQ=255)
                                                                                                                    gcaTGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTc                                                                 >  2:276232/1‑112 (MQ=255)
                                                                                                                    gcaTGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTc                                                                 <  1:276232/112‑1 (MQ=255)
                                                                                                                      aTGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAaca                           <  1:617929/148‑1 (MQ=255)
                                                                                                                      aTGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAaca                           >  2:617929/1‑148 (MQ=255)
                                                                                                                        gtgCCTGGCACAGGCCTGG‑CGCTGCGCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccgg                       <  2:449963/150‑1 (MQ=255)
                                                                                                                           ccTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAacaccggcac                    >  1:311530/1‑150 (MQ=255)
                                                                                                                             tGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGccc                                                                                           <  1:457696/77‑1 (MQ=255)
                                                                                                                             tGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGccc                                                                                           >  2:457696/1‑77 (MQ=255)
                                                                                                                                              cTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTc  >  1:307166/1‑150 (MQ=255)
                                                                                                                                              cTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTc  >  1:774632/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGAAGGCCTGGATACGATTGAGCATCAGGCCTGCCCCACCAACGCGCGCACCTGTGGGAGTTGCCAGAAGGCCTCGCGCACCGCCTGATCAGAGAAACGCTCACCCAGGCGCTGCAGCATGTGCCTGGCACAGGCCTGG‑CGCTGCCCAGCGTCCAGTACGGCCATGTGCTGCAGGCCCTGGGCCAGCTGCGCGGCGTCGCCCAGCGGGAACAGCACGCCAACGCCCTCGACCACTTCGCGGGCACCGCCACAGGCCGTGGCCAACACCGGCACACCGGCAACCATCGCTTC  >  NC_002947/5624657‑5624947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑