| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 289,947 | T→G | 11.4% | intergenic (+30/‑136) | oprE → / → PP_0235 | outer‑membrane porin E/peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 289,947 | 0 | T | G | 11.4% | 89.7 / 3.4 | 35 | intergenic (+30/‑136) | oprE/PP_0235 | outer‑membrane porin E/peroxidase |
| Reads supporting (aligned to +/- strand): ref base T (14/17); new base G (2/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
AAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATGAGCCTCAAAC > NC_002947/289807‑290095 | aaaGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtt > 1:493121/1‑150 (MQ=255) gCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtttttt < 2:660699/150‑1 (MQ=255) cGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTtata > 2:403342/1‑150 (MQ=255) aGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTAtaaataaa < 1:61440/150‑1 (MQ=255) gcggcgAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATaaataaat > 2:484308/1‑150 (MQ=255) aaaGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGttat < 1:484308/150‑1 (MQ=255) cccTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTc < 1:231193/150‑1 (MQ=255) gACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCttttt < 1:287362/150‑1 (MQ=255) aaaGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGtt > 2:617976/1‑150 (MQ=255) aaGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGttt < 1:110997/150‑1 (MQ=255) cAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGa > 1:250508/1‑76 (MQ=255) cAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGa < 2:250508/76‑1 (MQ=255) gACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCttttt > 2:764293/1‑139 (MQ=255) gACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCttttt < 1:764293/139‑1 (MQ=255) tCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTtgt > 1:316299/1‑82 (MQ=255) tCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTtgt < 2:316299/82‑1 (MQ=255) ttCATCGTGAACTACACTTACAACTTCCTGTAACCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAg > 1:104223/1‑150 (MQ=255) tCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGcac < 2:493121/150‑1 (MQ=255) ttACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCGGGCACATTGAACCCATAc < 1:617976/150‑1 (MQ=255) tACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACg < 1:335063/150‑1 (MQ=255) cTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCAATTGCAGGCACATTGAACCCATACGGTACa > 2:230284/1‑150 (MQ=255) tAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTa > 1:357339/1‑141 (MQ=255) tAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTa < 2:357339/141‑1 (MQ=255) aaGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAAcccag < 1:403342/150‑1 (MQ=255) gCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGttttt < 2:774706/105‑1 (MQ=255) gCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGttttt > 1:774706/1‑105 (MQ=255) tGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGcc > 1:755495/1‑150 (MQ=255) tGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGcc > 1:43646/1‑150 (MQ=255) aaaagCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGa < 2:528421/139‑1 (MQ=255) aaaagCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGa > 1:528421/3‑141 (MQ=255) aagCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTataaa > 1:66593/1‑61 (MQ=37) aagCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTataaa < 2:66593/61‑1 (MQ=37) cTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATGAGCCTCa > 2:540710/1‑150 (MQ=255) gATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATGAGCCTCaaa < 1:230284/150‑1 (MQ=255) aTGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATGAGCCTCAAAc < 1:540710/150‑1 (MQ=255) | AAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATGAGCCTCAAAC > NC_002947/289807‑290095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |