| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,674,716 | A→G | 13.8% | intergenic (+27/+114) | rhlE‑II → / ← yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,674,716 | 0 | A | G | 13.8% | 69.3 / 3.7 | 29 | intergenic (+27/+114) | rhlE‑II/yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Reads supporting (aligned to +/- strand): ref base A (11/14); new base G (2/2); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
ATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAG > NC_002947/5674579‑5674865 | aTCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGCGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGa < 1:64080/150‑1 (MQ=255)aTCATTCGAAAGGAGTCCAAGCTGGACCCTTTCCCTACGCCTCATCAACTGGATGACCTGCCAAGCCGCCCACGCGTTGAGCGCCCAGCGCTGGTGACCCGCAAGGGCTGTTAAAATTCCCGAGCGGGCTGCTTCGCAGCCCTATCACGa < 1:662032/150‑1 (MQ=255) aGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCag < 2:240392/150‑1 (MQ=255) gTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCt > 1:443772/1‑150 (MQ=255) tttCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCtt < 2:23242/150‑1 (MQ=255) aaCTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGc > 2:185355/1‑112 (MQ=255) aaCTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGc < 1:185355/112‑1 (MQ=255) aCTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCGGCCCTATCACGACACAAgg > 2:691492/1‑110 (MQ=255) aCTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCGGCCCTATCACGACACAAgg < 1:691492/110‑1 (MQ=255) aCTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGc < 2:327553/150‑1 (MQ=255) ggATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCtt < 2:581673/150‑1 (MQ=255) ccACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCa > 2:193812/1‑150 (MQ=255) gTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGc > 1:314244/1‑150 (MQ=255) gcgcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCaga < 2:269489/90‑1 (MQ=255) gcgcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCaga > 1:269489/1‑90 (MQ=255) cccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCt > 2:126500/1‑95 (MQ=255) cccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCt < 1:126500/95‑1 (MQ=255) ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGccc > 1:740529/1‑144 (MQ=255) ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGCGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGccc < 2:740529/144‑1 (MQ=255) tgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg < 1:108885/150‑1 (MQ=255) ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTCGA‑‑‑TCTCTACAGGAGCGGCCtt < 1:398838/81‑1 (MQ=255) ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTCGA‑‑‑TCTCTACAGGAGCGGCCtt > 2:398838/1‑81 (MQ=255) ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca > 2:169307/1‑150 (MQ=255) gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac < 1:193812/150‑1 (MQ=255) tgctTCGCAGCCCTATCACGACACAAGGTCGCTCCTGCAGAGGATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAACTTACTTCTGCTTCACACCTTCGACACTg > 1:173852/1‑150 (MQ=255) cgctTTGCGGCCCTATCACGACACAAGGCCGCTCCTGTAGAGA‑‑‑TCAAGAAATTCTCTGCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt > 1:707824/2‑149 (MQ=11) cgctTTGCGGCCCTATCACGACACAAGGCCGCTCCTGTAGAGA‑‑‑TCAAGAAATTCTCTGCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt < 2:707824/148‑1 (MQ=11) tCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa < 1:624777/141‑1 (MQ=255) tCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa > 2:624777/1‑141 (MQ=255) aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag > 2:118977/1‑150 (MQ=255) | ATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGA‑‑‑TCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAG > NC_002947/5674579‑5674865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |