Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,685,956 G→C 10.6% P331P (CCC→CCG) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,685,9560GC10.6% 141.6 / 8.6 47P331P (CCC→CCG) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base C (3/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

AAGTGCGTCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTT  >  NC_002947/5685807‑5686101
                                                                                                                                                     |                                                                                                                                                  
aaGTGCGTCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAg                                                                                                                                                    >  1:512069/1‑150 (MQ=255)
       tCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCa                                                                                                                                             <  1:743701/150‑1 (MQ=255)
               tcaATCGGCGGCTCTTCCTCCAGGTGCTTTAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAgcccttgc                                                                                                                                     >  1:743655/1‑143 (MQ=255)
                 aaTCGGCGGCTGTGCCTCGAGGGGCGGCGACCAAGGCTGCACATAGCTTGCGCAGCCGACAAACAGCGCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGGCCCGGCAACGCCTGCAGCCCTTGCCg                                                                                                                                   <  2:431855/150‑1 (MQ=255)
                 aaTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGACCTTGCCg                                                                                                                                   >  1:431855/1‑150 (MQ=255)
                  aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCgg                                                                                                                                  <  1:148132/150‑1 (MQ=255)
                  aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGAGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCgg                                                                                                                                  <  2:743655/150‑1 (MQ=255)
                                ctccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTc                                                                                                                    >  1:88160/1‑150 (MQ=255)
                                         ctgcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATc                                                                                                           >  1:227747/1‑150 (MQ=255)
                                            caaccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGcc                                                                                                        <  2:512069/150‑1 (MQ=255)
                                               ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                     <  1:279738/150‑1 (MQ=255)
                                               ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                     <  2:227747/150‑1 (MQ=255)
                                                    gCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGAt                                                                                                <  1:334031/150‑1 (MQ=255)
                                                               cTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgc                                                                                     >  2:92927/1‑150 (MQ=255)
                                                                ttGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgct                                                                                    <  2:235425/150‑1 (MQ=255)
                                                                      aGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAaca                                                                             >  1:210868/1‑150 (MQ=255)
                                                                        ccGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                           >  1:714294/1‑150 (MQ=255)
                                                                          gACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGtt                                                                         >  2:517219/1‑150 (MQ=255)
                                                                           aCAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCATTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                           >  2:200315/1‑147 (MQ=255)
                                                                           aCAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCATTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                           <  1:200315/147‑1 (MQ=255)
                                                                                 aGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGat                                                                  <  1:517219/150‑1 (MQ=255)
                                                                                  gCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCggg                                                                                                                                 <  2:584294/87‑1 (MQ=255)
                                                                                  gCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCggg                                                                                                                                 >  1:584294/1‑87 (MQ=255)
                                                                                      ggCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                             <  1:169256/150‑1 (MQ=255)
                                                                                              ccggcAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCa                                                     <  2:88160/150‑1 (MQ=255)
                                                                                                      aTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                       >  2:783904/1‑77 (MQ=255)
                                                                                                      aTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                       <  1:783904/77‑1 (MQ=255)
                                                                                                            aTGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCg                                       <  1:341114/150‑1 (MQ=255)
                                                                                                              ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                             <  2:657418/126‑1 (MQ=255)
                                                                                                              ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                             >  1:657418/1‑126 (MQ=255)
                                                                                                              ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGc                                     <  2:210868/150‑1 (MQ=255)
                                                                                                               gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcattc                                                            >  1:625003/1‑126 (MQ=255)
                                                                                                               gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcattc                                                            <  2:625003/126‑1 (MQ=255)
                                                                                                                 cAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCCGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAg                                  >  2:51175/1‑150 (MQ=255)
                                                                                                                          gcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCAATCCATATCCTTGGAATGCTGACAAAATCTGAAGAG‑TACATGCATCACCAGCTGGCGTATCTCGGCCAGGTCCGAGcc                         >  1:776306/1‑150 (MQ=255)
                                                                                                                                   ctgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCCGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGACCGGCAGGCt                >  1:235336/1‑150 (MQ=255)
                                                                                                                                      cAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGcc             <  2:452133/150‑1 (MQ=255)
                                                                                                                                        aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 <  2:464212/49‑1 (MQ=255)
                                                                                                                                        aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 >  1:464212/1‑49 (MQ=255)
                                                                                                                                        aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 >  1:66170/1‑49 (MQ=255)
                                                                                                                                        aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 <  2:66170/49‑1 (MQ=255)
                                                                                                                                             cccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCg      >  1:490322/1‑150 (MQ=255)
                                                                                                                                              ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAg                   >  2:46138/1‑136 (MQ=255)
                                                                                                                                              ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAg                   <  1:46138/136‑1 (MQ=255)
                                                                                                                                              ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt     >  2:487705/1‑150 (MQ=255)
                                                                                                                                              ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt     >  2:220493/1‑150 (MQ=255)
                                                                                                                                              ccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGTCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt     >  1:788803/1‑150 (MQ=255)
                                                                                                                                                 gCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGtttt  >  1:385284/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAGTGCGTCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTT  >  NC_002947/5685807‑5686101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑