Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,685,958 G→C 10.6% P331A (CCC→GCC) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,685,9580GC10.6% 138.8 / 7.2 47P331A (CCC→GCC) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base C (3/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCA  >  NC_002947/5685814‑5686107
                                                                                                                                                |                                                                                                                                                      
tCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCa                                                                                                                                                   <  1:743701/150‑1 (MQ=255)
        tcaATCGGCGGCTCTTCCTCCAGGTGCTTTAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAgcccttgc                                                                                                                                           >  1:743655/1‑143 (MQ=255)
          aaTCGGCGGCTGTGCCTCGAGGGGCGGCGACCAAGGCTGCACATAGCTTGCGCAGCCGACAAACAGCGCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGGCCCGGCAACGCCTGCAGCCCTTGCCg                                                                                                                                         <  2:431855/150‑1 (MQ=255)
          aaTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGACCTTGCCg                                                                                                                                         >  1:431855/1‑150 (MQ=255)
           aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCgg                                                                                                                                        <  1:148132/150‑1 (MQ=255)
           aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGAGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCgg                                                                                                                                        <  2:743655/150‑1 (MQ=255)
                         ctccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTc                                                                                                                          >  1:88160/1‑150 (MQ=255)
                                  ctgcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATc                                                                                                                 >  1:227747/1‑150 (MQ=255)
                                     caaccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGcc                                                                                                              <  2:512069/150‑1 (MQ=255)
                                        ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                           <  1:279738/150‑1 (MQ=255)
                                        ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                           <  2:227747/150‑1 (MQ=255)
                                             gCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGAt                                                                                                      <  1:334031/150‑1 (MQ=255)
                                                        cTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgc                                                                                           >  2:92927/1‑150 (MQ=255)
                                                         ttGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgct                                                                                          <  2:235425/150‑1 (MQ=255)
                                                               aGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAaca                                                                                   >  1:210868/1‑150 (MQ=255)
                                                                 ccGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                                 >  1:714294/1‑150 (MQ=255)
                                                                   gACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGtt                                                                               >  2:517219/1‑150 (MQ=255)
                                                                    aCAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCATTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                                 >  2:200315/1‑147 (MQ=255)
                                                                    aCAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCATTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                                 <  1:200315/147‑1 (MQ=255)
                                                                          aGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGat                                                                        <  1:517219/150‑1 (MQ=255)
                                                                           gCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCggg                                                                                                                                       >  1:584294/1‑87 (MQ=255)
                                                                           gCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCggg                                                                                                                                       <  2:584294/87‑1 (MQ=255)
                                                                               ggCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                                   <  1:169256/150‑1 (MQ=255)
                                                                                       ccggcAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCa                                                           <  2:88160/150‑1 (MQ=255)
                                                                                               aTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                             <  1:783904/77‑1 (MQ=255)
                                                                                               aTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                             >  2:783904/1‑77 (MQ=255)
                                                                                                     aTGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCg                                             <  1:341114/150‑1 (MQ=255)
                                                                                                       ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                                   >  1:657418/1‑126 (MQ=255)
                                                                                                       ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                                   <  2:657418/126‑1 (MQ=255)
                                                                                                       ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGc                                           <  2:210868/150‑1 (MQ=255)
                                                                                                        gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcattc                                                                  >  1:625003/1‑126 (MQ=255)
                                                                                                        gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcattc                                                                  <  2:625003/126‑1 (MQ=255)
                                                                                                          cAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCCGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAg                                        >  2:51175/1‑150 (MQ=255)
                                                                                                                   gcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCAATCCATATCCTTGGAATGCTGACAAAATCTGAAGAG‑TACATGCATCACCAGCTGGCGTATCTCGGCCAGGTCCGAGcc                               >  1:776306/1‑150 (MQ=255)
                                                                                                                            ctgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCCGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGACCGGCAGGCt                      >  1:235336/1‑150 (MQ=255)
                                                                                                                               cAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGcc                   <  2:452133/150‑1 (MQ=255)
                                                                                                                                 aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                       <  2:464212/49‑1 (MQ=255)
                                                                                                                                 aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                       >  1:464212/1‑49 (MQ=255)
                                                                                                                                 aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                       <  2:66170/49‑1 (MQ=255)
                                                                                                                                 aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                       >  1:66170/1‑49 (MQ=255)
                                                                                                                                      cccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCg            >  1:490322/1‑150 (MQ=255)
                                                                                                                                       ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAg                         >  2:46138/1‑136 (MQ=255)
                                                                                                                                       ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAg                         <  1:46138/136‑1 (MQ=255)
                                                                                                                                       ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt           >  2:487705/1‑150 (MQ=255)
                                                                                                                                       ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt           >  2:220493/1‑150 (MQ=255)
                                                                                                                                       ccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGTCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt           >  1:788803/1‑150 (MQ=255)
                                                                                                                                          gCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGtttt        >  1:385284/1‑150 (MQ=255)
                                                                                                                                                gCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTGCAGGGATTTATTGAGAATGAGCTGGCGGAGCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGGTTTCCTCCa  >  1:492189/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                      
TCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCA  >  NC_002947/5685814‑5686107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑