Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,685,967 G→C 7.9% R328G (CGC→GGC)  PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,685,9670GC 7.9% 153.0 / 2.1 49R328G (CGC→GGC) PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base C (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCAAA  >  NC_002947/5685824‑5686109
                                                                                                                                               |                                                                                                                                               
aaTCGGCGGCTGTGCCTCGAGGGGCGGCGACCAAGGCTGCACATAGCTTGCGCAGCCGACAAACAGCGCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGGCCCGGCAACGCCTGCAGCCCTTGCCg                                                                                                                                           <  2:431855/150‑1 (MQ=255)
aaTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGACCTTGCCg                                                                                                                                           >  1:431855/1‑150 (MQ=255)
 aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCgg                                                                                                                                          <  1:148132/150‑1 (MQ=255)
 aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGAGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCgg                                                                                                                                          <  2:743655/150‑1 (MQ=255)
               ctccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTc                                                                                                                            >  1:88160/1‑150 (MQ=255)
                        ctgcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATc                                                                                                                   >  1:227747/1‑150 (MQ=255)
                           caaccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGcc                                                                                                                <  2:512069/150‑1 (MQ=255)
                              ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                             <  1:279738/150‑1 (MQ=255)
                              ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                             <  2:227747/150‑1 (MQ=255)
                                   gCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGAt                                                                                                        <  1:334031/150‑1 (MQ=255)
                                              cTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgc                                                                                             >  2:92927/1‑150 (MQ=255)
                                               ttGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgct                                                                                            <  2:235425/150‑1 (MQ=255)
                                                     aGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAaca                                                                                     >  1:210868/1‑150 (MQ=255)
                                                       ccGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                                   >  1:714294/1‑150 (MQ=255)
                                                         gACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGtt                                                                                 >  2:517219/1‑150 (MQ=255)
                                                          aCAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCATTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                                   <  1:200315/147‑1 (MQ=255)
                                                          aCAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCATTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACg                                                                                   >  2:200315/1‑147 (MQ=255)
                                                                aGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGat                                                                          <  1:517219/150‑1 (MQ=255)
                                                                 gCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCggg                                                                                                                                         <  2:584294/87‑1 (MQ=255)
                                                                 gCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCggg                                                                                                                                         >  1:584294/1‑87 (MQ=255)
                                                                     ggCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                                     <  1:169256/150‑1 (MQ=255)
                                                                             ccggcAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCa                                                             <  2:88160/150‑1 (MQ=255)
                                                                                     aTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                               <  1:783904/77‑1 (MQ=255)
                                                                                     aTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                               >  2:783904/1‑77 (MQ=255)
                                                                                           aTGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCg                                               <  1:341114/150‑1 (MQ=255)
                                                                                             ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                                     >  1:657418/1‑126 (MQ=255)
                                                                                             ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcatt                                                                     <  2:657418/126‑1 (MQ=255)
                                                                                             ggCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGc                                             <  2:210868/150‑1 (MQ=255)
                                                                                              gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcattc                                                                    >  1:625003/1‑126 (MQ=255)
                                                                                              gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGattcattc                                                                    <  2:625003/126‑1 (MQ=255)
                                                                                                cAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCCGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAg                                          >  2:51175/1‑150 (MQ=255)
                                                                                                         gcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCAATCCATATCCTTGGAATGCTGACAAAATCTGAAGAG‑TACATGCATCACCAGCTGGCGTATCTCGGCCAGGTCCGAGcc                                 >  1:776306/1‑150 (MQ=255)
                                                                                                                  ctgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCCGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGACCGGCAGGCt                        >  1:235336/1‑150 (MQ=255)
                                                                                                                     cAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGcc                     <  2:452133/150‑1 (MQ=255)
                                                                                                                       aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                         <  2:464212/49‑1 (MQ=255)
                                                                                                                       aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                         <  2:66170/49‑1 (MQ=255)
                                                                                                                       aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                         >  1:464212/1‑49 (MQ=255)
                                                                                                                       aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                         >  1:66170/1‑49 (MQ=255)
                                                                                                                            cccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCg              >  1:490322/1‑150 (MQ=255)
                                                                                                                             ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAg                           >  2:46138/1‑136 (MQ=255)
                                                                                                                             ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAg                           <  1:46138/136‑1 (MQ=255)
                                                                                                                             ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt             >  2:487705/1‑150 (MQ=255)
                                                                                                                             ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt             >  2:220493/1‑150 (MQ=255)
                                                                                                                             ccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGTCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGt             >  1:788803/1‑150 (MQ=255)
                                                                                                                                gCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGtttt          >  1:385284/1‑150 (MQ=255)
                                                                                                                                      gCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTGCAGGGATTTATTGAGAATGAGCTGGCGGAGCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGGTTTCCTCCa    >  1:492189/1‑150 (MQ=255)
                                                                                                                                        tGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCaaa  <  2:490322/150‑1 (MQ=255)
                                                                                                                                        tGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCaaa  >  2:637076/1‑150 (MQ=255)
                                                                                                                                        tGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCaaa  <  1:92927/150‑1 (MQ=255)
                                                                                                                                               gTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTga                                                        <  2:410629/89‑2 (MQ=255)
                                                                                                                                               gTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTga                                                        >  1:410629/1‑88 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTG‑CAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCAAA  >  NC_002947/5685824‑5686109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑