Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,793,943 A→T 12.9% L1338Q (CTG→CAG)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,793,9430AT12.9% 79.1 / 5.4 31L1338Q (CTG→CAG) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (17/10);  new base T (2/2);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

AGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGGCGTCGCCTTCCAGGTG  >  NC_002947/5793795‑5794091
                                                                                                                                                    |                                                                                                                                                    
aGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAg                                                                                                                                                     >  1:76183/1‑150 (MQ=255)
                    tCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTg                                                                                                                                                 <  2:240560/134‑1 (MQ=255)
                    tCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTg                                                                                                                                                 >  1:240560/1‑134 (MQ=255)
                    tCTTGCCCAGAACGCAGACAAAGCCGCCGGGCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGt                                                                                                                                 >  1:598184/1‑150 (MQ=255)
                        gCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTAcag                                                                                                                             >  2:16979/1‑150 (MQ=255)
                           cAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTAcaggca                                                                                                                          >  2:583288/1‑150 (MQ=255)
                                     aCAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCcg                                                                                                                >  2:66748/1‑150 (MQ=255)
                                               cgGTCATGTATTCACAGCAGTGATCGACAGTGCCCTCGACAACAGCATGGGCGCCGTAGTTAGGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGgcgc                                                                                                      >  2:47571/1‑150 (MQ=255)
                                                ggTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAGCTGTTTgccgcag                                                                                                                                           >  2:735809/1‑110 (MQ=255)
                                                ggTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAGCTGTTTgccgcag                                                                                                                                           <  1:735809/112‑3 (MQ=255)
                                                ggTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAGCTGTTTgccgcag                                                                                                                                           <  1:735804/112‑3 (MQ=255)
                                                ggTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAGCTGTTTgccgcag                                                                                                                                           >  2:735804/1‑110 (MQ=255)
                                                      gTATTCACAGCAGTGATCGCCAGTGCAAGCTGTAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTg                                                                                               >  1:20601/1‑150 (MQ=255)
                                                            acaGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTgcc                                                                                                                                               <  1:105455/96‑1 (MQ=255)
                                                            acaGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTgcc                                                                                                                                               >  2:105455/1‑96 (MQ=255)
                                                               gcagTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTCGCACCGTACACGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGaa                                                                                      <  2:598184/150‑1 (MQ=255)
                                                                      tCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTg                                                                               >  1:371968/1‑150 (MQ=255)
                                                                         ccAGTGCCCTCGACAACAGCATGGACGGTGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCg                                                                            >  1:222857/1‑150 (MQ=255)
                                                                                          aGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGt                                                           >  1:312336/1‑150 (MQ=255)
                                                                                           gCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTg                                                          <  2:312336/150‑1 (MQ=255)
                                                                                                   gcCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGcc                                                  >  1:790249/1‑150 (MQ=255)
                                                                                                                  aGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCttt                                   <  1:16979/150‑1 (MQ=255)
                                                                                                                           cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCtt                                                     <  2:112510/123‑1 (MQ=255)
                                                                                                                           cTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCtt                                                     >  1:112510/1‑123 (MQ=255)
                                                                                                                                 cGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGTCGTTGTTTCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGtt                    <  2:222857/150‑1 (MQ=255)
                                                                                                                                    aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGAc                                                        <  1:71817/111‑1 (MQ=255)
                                                                                                                                    aGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGAc                                                        >  2:71817/1‑111 (MQ=255)
                                                                                                                                          tGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGGCGTCGcc           <  1:66748/150‑1 (MQ=255)
                                                                                                                                              gCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGGCGTCGCCTTcc       >  2:617391/1‑150 (MQ=255)
                                                                                                                                                 aaCAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGGCGTCGCCTTCCAgg    <  2:371968/150‑1 (MQ=255)
                                                                                                                                                   cAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGGCGTCGCCTTCCAGGTg  >  1:628831/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGAACCGAAGTTGTAACCGGTCTTGCCCAGAACGCAGACAAAGCCGCCGGTCATGTATTCACAGCAGTGATCGCCAGTGCCCTCGACAACAGCATGGGCGCCGGAGTTACGTACAGCGAAGCGCTCGCCCGCAGTACCTGCGGCAAACAGCTTGCCGCCAGTGGCACCGTACAGGCAGGTGTTGCCGACGATGGCGCTGTGCTGGGTTTCGAACGGGCTGCCGGCTGGCGGCACGATGGTGACCTTGCCACCGGTCATGCCTTTGCCGACGTAATCGTTGGCGTCGCCTTCCAGGTG  >  NC_002947/5793795‑5794091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑