Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,839,742 T→A 10.0% intergenic (+63/+40) yheT → / ← sseA putative hydrolase/3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,839,7420TA10.0% 117.0 / 4.3 40intergenic (+63/+40)yheT/sseAputative hydrolase/3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (19/17);  new base A (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TCGATGGTAGCCTGCGAAATCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCCGCCAGCGCCAGCGCAAACAG  >  NC_002947/5839606‑5839886
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tCGATGGTAGCCTGCGAAATCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCAATGCCg                                                                                                                                             >  1:238033/1‑142 (MQ=255)
tCGATGGTAGCCTGCGAAATCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCAATGCCg                                                                                                                                             <  2:238033/142‑1 (MQ=255)
  gATGGTAGCCTGCGAAATCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCAATGCCGGTgtccgcgg                                                                                                                                   <  2:555322/150‑8 (MQ=255)
            tGCGAAATCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGTCATTGCCGGTGCCACCCACCGCGGGGGt                                                                                                                         <  1:744052/150‑1 (MQ=255)
                   tCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAAccc                                                                                                                  >  1:684843/1‑150 (MQ=255)
                         gTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTcag                                                                                                            >  1:676784/1‑148 (MQ=255)
                           actactTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCt                                                                                                          <  1:299515/150‑1 (MQ=255)
                             tactTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCgg                                                                                                                             <  1:290167/129‑1 (MQ=255)
                             tactTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCgg                                                                                                                             >  2:290167/1‑129 (MQ=255)
                              actTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTAc                                                                                                       <  1:497809/150‑1 (MQ=255)
                                ttGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGt                                                                                                                         >  2:444824/1‑130 (MQ=255)
                                ttGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGt                                                                                                                         <  1:444824/130‑1 (MQ=255)
                                      tccgatcttCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTCAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGt                                                                                               <  1:571924/143‑1 (MQ=255)
                                       gacgGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTg                                                                                              >  1:663726/1‑150 (MQ=255)
                                       gacgGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTg                                                                                              >  2:340971/1‑150 (MQ=255)
                                       gacgGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTg                                                                                              <  2:351373/150‑1 (MQ=255)
                                             ttCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCACGCTGGCACCCGCATTACCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCAcc                                                                                        >  1:412874/1‑150 (MQ=255)
                                                             ggACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGgttgt                                                                        <  2:684843/150‑1 (MQ=255)
                                                             ggACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGgttgt                                                                        <  2:330411/150‑1 (MQ=255)
                                                              gACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGgttgtt                                                                       >  1:302078/1‑150 (MQ=255)
                                                                 ggTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGt                                                                                                               >  2:138027/1‑107 (MQ=255)
                                                                 ggTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGt                                                                                                               <  1:138027/107‑1 (MQ=255)
                                                                 ggTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCgg                                                                             <  2:543893/141‑1 (MQ=255)
                                                                 ggTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCgg                                                                             >  1:543893/1‑141 (MQ=255)
                                                                      ctagcCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGgttg                                                                         <  1:659037/140‑1 (MQ=255)
                                                                      ctagcCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGgttg                                                                         >  2:659037/1‑140 (MQ=255)
                                                                           ccGCGAATTGGTAAAGGCTTCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCt                                                                                                          >  1:18015/1‑102 (MQ=255)
                                                                           ccGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCt                                                                                                          <  2:18015/102‑1 (MQ=255)
                                                                            cgcgAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTcc                                                         >  1:450376/1‑150 (MQ=255)
                                                                                     gTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGa                                                                     <  2:466622/129‑1 (MQ=255)
                                                                                     gTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGa                                                                     >  1:466622/1‑129 (MQ=255)
                                                                                         aGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATa                                            <  2:663726/150‑1 (MQ=255)
                                                                                          ggCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGACTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATAc                                           >  2:226578/1‑150 (MQ=255)
                                                                                             tGCGGGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGc                                        <  2:363588/150‑1 (MQ=255)
                                                                                                            ggAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTAc                         <  2:450376/150‑1 (MQ=255)
                                                                                                                cGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCcg                     >  2:457700/1‑150 (MQ=255)
                                                                                                                       gCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCcgccagcgc              >  2:600852/1‑150 (MQ=255)
                                                                                                                               gcacCGGCAATGCCGGTGTCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCCGCCAGCGCCAGCGCaa      >  2:323476/2‑150 (MQ=255)
                                                                                                                                   cGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCCGCCAGCGCCAGCGCAAACAg  >  1:162105/1‑150 (MQ=255)
                                                                                                                                   cGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGAGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCCGCCAGCGCCAGCGCAAACAg  >  2:515928/1‑150 (MQ=255)
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TCGATGGTAGCCTGCGAAATCCGGGGTACTACTTGGAACGACGGATTCCGCAGTGGCTGGTGGACGGTAGCTAGCCCGCGAATTGGTAAAGGCTGCGCGCCCCCTGTGGGAGCGGGCATGCCCGCGAACACCGGCATTGCCGGTGCCACCCACCGCGGGGGTTCAACCCGGTCTGCTTACTCGCCGGTGGCCACCGCATGCTGCGGGTTGTTGATCCACTCACTCCACGACCCCGCATACAGCTTGCCCAGCGGGTACCCCGCCAGCGCCAGCGCAAACAG  >  NC_002947/5839606‑5839886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑