Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,872,077 T→A 8.3% D211V (GAC→GTC)  ptsP ← phosphoenolpyruvate‑dependent regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,872,0770TA 8.3% 145.6 / 4.2 48D211V (GAC→GTC) ptsPphosphoenolpyruvate‑dependent regulator
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GTCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACGGATC  >  NC_002947/5871935‑5872223
                                                                                                                                              |                                                                                                                                                  
gTCTTCAAGCATCATCAGGTAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGAc                                                                                                                                             >  2:95406/1‑150 (MQ=255)
  cTTCAAGCATCATCAGGTAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACgc                                                                                                                                           <  1:95406/150‑2 (MQ=255)
   ttCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGt                                                                                                                                          >  1:253315/1‑150 (MQ=255)
        gCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCAAACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGt                                                                                                                                     >  2:706816/1‑150 (MQ=255)
           tcatcaGATAGACGTCGAACAGCGCACGCTCTTCCGGGTGCAGCTGGTTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCg                                                                                                                                  >  1:416251/1‑150 (MQ=255)
              tcaGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACg                                                                                                                                            >  2:569809/1‑137 (MQ=255)
              tcaGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACg                                                                                                                                            <  1:569809/137‑1 (MQ=255)
              tcaGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGa                                                                                                                               >  1:313809/1‑150 (MQ=255)
                       cGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCa                                                                                                                      <  2:97348/150‑1 (MQ=255)
                        gTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAg                                                                                                                     <  2:493028/150‑1 (MQ=255)
                           gAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTc                                                                                                                  >  1:52499/1‑150 (MQ=255)
                            aaCAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTcg                                                                                                                 >  2:539326/1‑150 (MQ=255)
                              cAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCGAGCGTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTcggc                                                                                                               <  2:62950/150‑1 (MQ=255)
                                  gcACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTcggccggc                                                                                                           >  1:746070/1‑150 (MQ=255)
                                  gcACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTcggccggc                                                                                                           >  1:579290/1‑150 (MQ=255)
                                     cGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCggcggc                                                                                                        <  1:539326/150‑1 (MQ=255)
                                        tctTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAAc                                                                                                     <  2:579290/150‑1 (MQ=255)
                                         ctTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACa                                                                                                    <  2:52499/150‑1 (MQ=255)
                                                       tGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGc                                                                                      <  1:272519/150‑1 (MQ=255)
                                                             gCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCgg                                                                                                                                 >  1:443896/1‑101 (MQ=255)
                                                             gCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCgg                                                                                                                                 <  2:443896/101‑1 (MQ=255)
                                                              ccAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGa                                                                                                                                              <  1:110064/87‑1 (MQ=255)
                                                              ccAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGa                                                                                                                                              >  2:110064/1‑87 (MQ=255)
                                                                     ggCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGa                                                                                                                               >  1:218134/1‑95 (MQ=255)
                                                                     ggCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGa                                                                                                                               <  2:218134/95‑1 (MQ=255)
                                                                            aGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGaa                                                                 >  1:238928/1‑150 (MQ=255)
                                                                             gCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGTCCGCCCCACCGCGGCACCAGGCGAAc                                                                <  1:706816/150‑1 (MQ=255)
                                                                               ttGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAAccc                                                              <  2:746070/150‑1 (MQ=255)
                                                                                tGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCg                                                             >  1:354818/1‑150 (MQ=255)
                                                                                   gcATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCcggcg                                                                                                          <  2:377521/102‑1 (MQ=255)
                                                                                   gcATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCcggcg                                                                                                          >  1:377521/1‑102 (MQ=255)
                                                                                           tcgCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAg                                                                      >  2:378433/1‑130 (MQ=255)
                                                                                           tcgCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAg                                                                      <  1:378433/130‑1 (MQ=255)
                                                                                               gcACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAgc                                              >  1:330609/1‑150 (MQ=255)
                                                                                                     cTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGGACCCGGCACACCCACGAAGCGCGCtt                                        >  1:598981/1‑150 (MQ=255)
                                                                                                          aGGTCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCAACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACAccc                                                     <  2:409505/132‑1 (MQ=255)
                                                                                                          aGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCAACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACAccc                                                     >  1:409505/1‑132 (MQ=255)
                                                                                                           gggCGTGCTGTAACGGCTTTAGTTCGGTGTCGATGTCATCGACGGTCTTGTCCTGTACCACTTCCAGGTCGGCCGGCGGCAACAGCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAATCGCGCTTCGTgtg                                  <  2:21881/150‑3 (MQ=255)
                                                                                                              cGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATAc                               <  2:330609/150‑1 (MQ=255)
                                                                                                                   tGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg                          >  2:649335/1‑150 (MQ=255)
                                                                                                                   tGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg                          >  2:591477/1‑150 (MQ=255)
                                                                                                                    ggAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGc                         <  2:416251/150‑1 (MQ=255)
                                                                                                                       aCAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCt                      <  2:253315/150‑1 (MQ=255)
                                                                                                                                 ttCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGACCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAg            >  1:494771/1‑150 (MQ=255)
                                                                                                                                  tCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGGCCTGACGGCCCAgg           <  2:238928/150‑1 (MQ=255)
                                                                                                                                    accGTCGATGACATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg                          <  1:114937/131‑1 (MQ=255)
                                                                                                                                    accGTCGATGACATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg                          >  2:114937/3‑133 (MQ=255)
                                                                                                                                           aTGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACGGATc  <  1:649335/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GTCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACGGATC  >  NC_002947/5871935‑5872223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑