| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,914,180 | C→T | 20.0% | intergenic (+16/+137) | spuI → / ← argA | glutamylpolyamine synthetase/amino acid N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,914,180 | 0 | C | T | 20.0% | 69.9 / 12.8 | 35 | intergenic (+16/+137) | spuI/argA | glutamylpolyamine synthetase/amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (11/17); new base T (4/3); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCC > NC_002947/5914050‑5914312 | gTACATCGATGCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgt < 1:431979/150‑1 (MQ=255) aTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTCAATGGGTACATGGTGGACGAAAACGCGGCCCCTCAAGGGCGGCGTTTTTGTTTga > 2:222436/1‑150 (MQ=255) aTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga < 2:59144/150‑1 (MQ=255) aTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga < 2:331893/150‑1 (MQ=255) aTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCCGCGTTTCTGCTTga > 2:411694/1‑150 (MQ=255) gAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGCAAACAAAAACGCCGCCCTTGAGGGGCGGCGttttc > 2:380928/1‑135 (MQ=38) gAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGCAAACAAAAACGCCGCCCTTGAGGGGCGGCGttttc < 1:380928/136‑2 (MQ=38) gTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgtttg < 2:796522/117‑1 (MQ=255) gTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgtttg > 1:796522/1‑117 (MQ=255) tGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGc < 1:80659/150‑1 (MQ=255) aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGcc < 1:317255/127‑1 (MQ=255) aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGcc > 2:317255/1‑127 (MQ=255) aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCgg > 2:347680/1‑137 (MQ=255) aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCgg < 1:347680/137‑1 (MQ=255) aGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgttt < 2:580250/111‑1 (MQ=255) aGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgttt > 1:580250/1‑111 (MQ=255) ggAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcggc > 1:295436/1‑100 (MQ=255) ggAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcggc < 2:295436/100‑1 (MQ=255) gAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAg < 2:637657/93‑1 (MQ=255) gAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAg > 1:637657/1‑93 (MQ=255) agagTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGaa < 1:411694/150‑1 (MQ=255) gTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGGCGttttc > 2:22849/1‑87 (MQ=25) gTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGGCGttttc < 1:22849/88‑2 (MQ=25) ttCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgttt > 2:760462/1‑91 (MQ=255) ttCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTCTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgttt < 1:760462/91‑1 (MQ=255) tCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTg < 2:741042/150‑1 (MQ=255) gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGc > 2:452233/1‑115 (MQ=255) gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGc < 1:452233/115‑1 (MQ=255) cAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCCAAATCCTGTGACTTACCTGTGGGAGCggg < 1:222436/150‑1 (MQ=255) aCTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAg < 2:445379/123‑1 (MQ=255) aCTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAg > 1:445379/1‑123 (MQ=255) gCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGaa < 1:501028/150‑1 (MQ=255) aaaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGccc > 2:234866/1‑150 (MQ=39) aaaCGCCGCCCTTGAGGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCa < 2:481503/75‑1 (MQ=37) aaaCGCCGCCCTTGAGGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCa > 1:481503/1‑75 (MQ=37) | GTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCC > NC_002947/5914050‑5914312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |