| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,914,280 | T→C | 14.3% | intergenic (+116/+37) | spuI → / ← argA | glutamylpolyamine synthetase/amino acid N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,914,280 | 0 | T | C | 14.3% | 70.4 / 3.9 | 28 | intergenic (+116/+37) | spuI/argA | glutamylpolyamine synthetase/amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (14/10); new base C (2/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
ACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCGGCAGCCGCTCCACGCCACTCGGCGCAAAGCCGCGCTCGCGGAACCA > NC_002947/5914139‑5914427 | ccggcggcgcgggcttcgctgtacaactaccagcgcaattcgaagattttcgagaagcccctgtaagccttggcCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCgg > 1:658406‑M1/75‑150 (MQ=255)ccggcggcgcgggcttcgctgtacaactaccagcgcaattcgaagattttcgagaagcccctgtaagcctgggtCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCgg > 1:793599‑M1/75‑150 (MQ=255) cAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCCAAATCCTGTGACTTA‑CCTGTGGGAGCggg < 1:222436/150‑1 (MQ=255) gCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGaa < 1:501028/150‑1 (MQ=255) aaaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGccc > 2:234866/1‑150 (MQ=39) tCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCCGGGTAATTTCGAAAGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCGtct > 2:589350/1‑150 (MQ=255) cggcgTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAAt > 2:673710/1‑150 (MQ=255) ggcgTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATc > 2:376986/1‑150 (MQ=255) gagaagcccctgtaagcctgggcCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGa > 2:536050‑M1/24‑95 (MQ=255) gagaagcccctgtaagcctgggcCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGACTGTGACTTA‑CCTGTGGGa < 1:536050‑M1/72‑1 (MQ=255) cccctgtaagcctgggcCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTgg < 2:122836‑M1/70‑1 (MQ=255) cccctgtaagcctgggcCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTgg > 1:122836‑M1/18‑87 (MQ=255) tgAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAgg > 1:431436/1‑105 (MQ=255) tgAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAgg < 2:431436/105‑1 (MQ=255) gtgtCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTgc > 2:565727/1‑140 (MQ=255) gtgtCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTgc < 1:565727/140‑1 (MQ=255) CCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCgg < 2:789177‑M1/150‑1 (MQ=255) TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAgg > 2:537729‑M1/1‑69 (MQ=255) TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAgg < 1:537729‑M1/69‑1 (MQ=255) CCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCtt < 2:423380‑M1/102‑1 (MQ=255) CCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCtt > 1:423380‑M1/1‑102 (MQ=255) tAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGCGGGAGCGGGCGTGCCcg < 1:552686/51‑1 (MQ=255) tAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGCGGGAGCGGGCGTGCCcg > 2:552686/1‑51 (MQ=255) cTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCGGCAGCCGCTCCAc < 2:319726/136‑1 (MQ=255) cTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCGGCAGCCGCTCCAc > 1:319726/1‑136 (MQ=255) aaGCCTGTGACTTACCCTGCGGGAGCGGGCGTGCCCGCGAAGAGggc > 2:569065/1‑47 (MQ=11) aaGCCTGTGACTTACCCTGCGGGAGCGGGCGTGCCCGCGAAGAGggc < 1:569065/47‑1 (MQ=11) ttA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCGGCAGCCGCTCCACGCCACTCGGCGCAAAGCCGCGCTCGCgg > 2:254745/1‑150 (MQ=255) tgtgGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCGGCAGCCGCTCCACGCCACTCGGCGCAAAGCCGCGCTCGCGGAACca > 2:471495/1‑150 (MQ=255) | ACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTA‑CCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGCTTCTCGAAAATCTTCGAATTGCGCTGGTAGTTGTACAGCGAAGCCCGCGCCGCCGGCAGCCGCTCCACGCCACTCGGCGCAAAGCCGCGCTCGCGGAACCA > NC_002947/5914139‑5914427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |