| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,958,085 | A→C | 8.9% | Y478S (TAC→TCC) | cyaA → | adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,958,085 | 0 | A | C | 8.9% | 118.9 / 2.2 | 44 | Y478S (TAC→TCC) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base C (2/2); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CTCAATGTGCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGACAGCAGCACCCGCATGGCGCTGCATCCA > NC_002947/5957940‑5958233 | cTCAATGTGCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCAc > 1:725504/1‑150 (MQ=255) aaTGTGCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCGAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTAcccatgc < 1:27662/150‑6 (MQ=255) aaTGTGCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTAc < 2:372649/144‑1 (MQ=255) aaTGTGCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTAc > 1:372649/1‑144 (MQ=255) gtgCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAg < 1:510044/150‑1 (MQ=255) cGGCGCCTGTACGCCGCCTTCGAGCGCCGCTCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTCCCAGTGGTAGCTgc > 1:146418/1‑144 (MQ=255) cGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTCCCAGTGGTAGCTgc < 2:146418/145‑2 (MQ=255) tACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTgg < 1:341590/129‑1 (MQ=255) tACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTgg > 2:341590/1‑129 (MQ=255) gccgccTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTCCCAGTGGTAGCTgc > 1:446294/1‑132 (MQ=255) gccgccTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACa > 2:776982/1‑136 (MQ=255) gccgccTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACa < 1:776982/136‑1 (MQ=255) gccgccTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCCGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTCCTAGTGGTAGCTgc < 2:446294/133‑2 (MQ=255) cGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGa < 2:307320/150‑1 (MQ=255) gAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAg < 2:626956/120‑1 (MQ=255) gAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAg > 1:626956/1‑120 (MQ=255) aGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAgg < 2:544579/150‑1 (MQ=255) ccgcgccgGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTgg > 1:458288/1‑112 (MQ=255) ccgcgccgGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTgg < 2:458288/112‑1 (MQ=255) cgcgccgGCAAGGTCGAAGGTATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAg < 2:326595/150‑1 (MQ=255) aTTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAgcgc < 2:341410/150‑1 (MQ=255) cGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGa < 1:573614/150‑1 (MQ=255) tCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTAc < 1:473193/150‑1 (MQ=255) cccTGACCTCGCCGAAGACACCCTGAAGGTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGa > 1:543570/1‑150 (MQ=255) ccTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAg < 2:242461/90‑1 (MQ=255) ccTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAg > 1:242461/1‑90 (MQ=255) acCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCAt > 1:739056/1‑150 (MQ=255) acCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACACATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCAt > 1:773267/1‑150 (MQ=255) ccTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCg > 2:405622/1‑150 (MQ=255) ccTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCg > 1:272301/1‑150 (MQ=255) ccTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCACCCGGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCg > 1:373663/1‑150 (MQ=255) tGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTgg > 1:617316/1‑134 (MQ=255) tGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTgg < 2:617316/134‑1 (MQ=255) tCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGa > 1:271898/1‑150 (MQ=255) ccAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGAt < 2:271898/150‑1 (MQ=255) ggCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGa < 1:528750/150‑1 (MQ=255) ccAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGAcagca < 1:405622/150‑1 (MQ=255) cGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCg < 2:349796/125‑1 (MQ=255) cGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCg > 1:349796/1‑125 (MQ=255) cGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGAca < 1:665190/142‑1 (MQ=255) cGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGAca > 2:665190/1‑142 (MQ=255) tACCCCTGGGAGCTGTACAGCGGCAACCTGAGCGCCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGAGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGAc < 2:264185/123‑1 (MQ=255) tACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTTCCGAGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGAc > 1:264185/1‑123 (MQ=255) tACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGACAGCAGCACCCGCATGGCGCTGCATCCa > 2:671561/1‑150 (MQ=255) | CTCAATGTGCTGGGCCGGCGCCTGTACGCCGCCTTCGAGCGCCGCGCCGGCAAGGTCGAAGTGATTAACCCCGGCATCGCCCCTGACCTCGCCGAAGACACCCTGACGCTGGTCCAGGCACCCAACCGCAAGGAGCCGGGCAGCTACCACTGGGAGCTGTACAGCGGCAACCTGAGCACCCATGAGGTACAGCACTTCAGCCCGATGAAGCGCTGCCGCGAGCTACTCGAACTGCTGACCTGGGCCCATCGCAACGGGGTGATCGACAGCAGCACCCGCATGGCGCTGCATCCA > NC_002947/5957940‑5958233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |