| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,988,881 | G→T | 32.8% | intergenic (+119/‑427) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,881 | 0 | G | T | 32.8% | 107.4 / 30.8 | 52 | intergenic (+119/‑427) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (16/18); new base T (7/10); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGG > NC_002947/5988743‑5989029 | ttGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑Gc < 1:518959/150‑1 (MQ=255) gCCTTTTGGCGCGTGCCTTGAGGGCTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCACTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACCCCTCGata > 2:775588/1‑150 (MQ=255) cTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGAcgc < 1:569125/103‑1 (MQ=255) cTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGAcgc > 2:569125/1‑103 (MQ=255) tGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg < 2:748321/150‑1 (MQ=255) gTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTac < 1:278854/135‑1 (MQ=255) gTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTac > 2:278854/1‑135 (MQ=255) aGTGCCTGTGAGATTGGGCGCTGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc < 2:409060/150‑1 (MQ=255) tgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCt > 1:411328/1‑148 (MQ=255) tgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCt < 2:411328/148‑1 (MQ=255) gcggagcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAg > 2:389578/6‑128 (MQ=2) gcggagcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAg < 1:389578/123‑1 (MQ=2) gagcgcCGCCCGCGCGGCGCTTCGCGGGTAACCCCGCTCCTACGTTTGTTTCGGCCCCATGTTTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCagga < 1:529771/148‑1 (MQ=2) ggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggag < 2:363920/150‑1 (MQ=255) ggCGCCGCCCGCGCGGCGCTTCGCGCGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGGCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggag < 1:57143/150‑1 (MQ=14) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGTCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagg < 1:504982/150‑1 (MQ=255) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGcgtcgt > 2:283327/1‑114 (MQ=255) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGcgtcgt < 1:283327/114‑1 (MQ=255) gcgcCGCCCCCGCGGCACTTCGCGGGAAAACCCGCCCCTACGTTTGTTTCGTGCCAATTATCCCTGCGGCATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTAGATATCGCGGCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggagg < 2:570001/150‑1 (MQ=2) cgccggcggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTgg < 1:93247/145‑1 (MQ=255) cccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGGTTGTGTGAGGCCAGTTATTCCTGTGGGACGCGCAGGCGAAATTTACCCCAGCGAGCG‑‑‑CGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagatcggaa > 1:779328/1‑143 (MQ=16) cggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGccgaaa < 2:93414/150‑5 (MQ=9) ttCGCGGGTAACCCCGCTCCTACGTTCGTTACCGGCAACTTAGGGCTGGGGGACGCGCACGCGA‑‑‑GCCCCATGTCCCGCGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 1:775588/150‑1 (MQ=21) gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGAGTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTAGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAg > 2:47604/1‑150 (MQ=9) cgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTACTGTGGGACCCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGAGGCTCGCGGCTGGCACGGGAGGAATTGACCAGaaaaaaaccgag > 2:324207/1‑146 (MQ=25) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAtt < 2:415183/131‑1 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAtt > 1:415183/1‑131 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaa > 2:654768/1‑143 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaa < 1:654768/143‑1 (MQ=255) gttAAACCCGCTCCTACGTTTGTTTCGGGCCACATGATTAAGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAg < 1:439329/148‑1 (MQ=2) gTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAg > 2:424708/1‑150 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac > 2:522436/1‑142 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 1:522436/142‑1 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGc > 2:455437/1‑150 (MQ=255) aaaCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTCCGGGCTTTGCGCGCCC‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCgg < 1:398404/150‑1 (MQ=2) aCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCTTACAAACAATGCg < 2:33183/97‑1 (MQ=2) aCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCg > 1:33183/1‑97 (MQ=12) cccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt < 1:687689/150‑1 (MQ=255) ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGtt > 1:118842/1‑150 (MQ=255) gCTCCTACGTTTTTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc < 2:368434/150‑1 (MQ=2) gCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc < 2:77554/150‑1 (MQ=2) tCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTgg < 1:67752/106‑1 (MQ=255) tCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTgg > 2:67752/1‑106 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 2:440426/131‑1 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac > 1:440426/1‑131 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAg < 1:400833/135‑1 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAg > 2:400833/1‑135 (MQ=255) tACGTTTGTTTCGGGCAAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcgcg > 2:469513/1‑150 (MQ=2) gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgc < 1:770105/90‑1 (MQ=255) gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgc > 2:770105/1‑90 (MQ=255) gtttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgcc > 1:609278/1‑150 (MQ=9) tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccg > 2:436596/1‑150 (MQ=9) gggCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGGCTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCcgcgcg > 1:155630/1‑150 (MQ=2) aCGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATg > 1:674720/1‑150 (MQ=255) cgcgCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATgg < 1:424708/150‑1 (MQ=255) | TTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGG > NC_002947/5988743‑5989029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |